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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated HEXIM1 Polyclonal antibody

HEXIM1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15676

Synonyms

HEXIM 1, Hexamethylene bis-acetamide-inducible protein 1, Estrogen down-regulated gene 1 protein, EDG1, CLP1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated HEXIM1 Polyclonal an (CL488-15676)

    IF Staining of HeLa using CL488-15676

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 HEXIM1 antibody (CL488-15676) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated HEXIM1 Polyclonal an (CL488-15676)

    FC experiment of HeLa using CL488-15676

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human HEXIM1 (CL488-15676) (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-15676 targets HEXIM1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HEXIM1 fusion protein Ag8144
Full Name hexamethylene bis-acetamide inducible 1
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC006460
Gene Symbol HEXIM1
Gene ID (NCBI) 10614
RRIDAB_2919122
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HEXIM1, also named CLP1, EDG1, HIS1 or MAQ1, is best known as the inhibitor of positive transcription elongation factor b, which regulates the transcription elongation of RNA polymerase II and controls 60-70% of mRNA synthesis. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Previous studies showed that HEXIM1 interacts with two key p53 regulators, nucleophosmin and HDM2, implying a possible connection between HEXIM1 and the p53 signaling pathway.The mainstream MW of this protein is 70 and 54 kDa. Catalog#15676-1-AP recognises both 70 and 54 kDa band.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 HEXIM1 antibody CL488-15676Download protocol
FC protocol for CL Plus 488 HEXIM1 antibody CL488-15676Download protocol
Standard Protocols
Click here to view our Standard Protocols