HEXIM1 Polyclonal antibody

• Featured Product
• KD/KO Validated

HEXIM1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 15676-1-AP

Synonyms

HEXIM 1, Hexamethylene bis-acetamide-inducible protein 1, Estrogen down-regulated gene 1 protein, EDG1, CLP1

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Validation Data Gallery

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  WB analysis using 15676-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15676-1-AP (HEXIM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15676-1-AP

  WB result of HEXIM1 antibody (15676-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HEXIM1 transfected HeLa cells.

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  IP experiment of HeLa using 15676-1-AP

  IP result of anti-HEXIM1 (IP:15676-1-AP, 4ug; Detection:15676-1-AP 1:5000) with HeLa cells lysate 1880 ug.

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  IHC staining of human breast cancer using 15676-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15676-1-AP (HEXIM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15676-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15676-1-AP (HEXIM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15676-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEXIM1 antibody (15676-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Product Information

15676-1-AP targets HEXIM1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HEXIM1 fusion protein Ag8144
• Full Name: hexamethylene bis-acetamide inducible 1
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC006460
• Gene Symbol: HEXIM1
• Gene ID (NCBI): 10614
• RRID: AB_2248363
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HEXIM1, also named CLP1, EDG1, HIS1 or MAQ1, is best known as the inhibitor of positive transcription elongation factor b, which regulates the transcription elongation of RNA polymerase II and controls 60-70% of mRNA synthesis. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Previous studies showed that HEXIM1 interacts with two key p53 regulators, nucleophosmin and HDM2, implying a possible connection between HEXIM1 and the p53 signaling pathway.The mainstream MW of this protein is 65-70 kDa and 54 kDa ( PMID: 33627647, PMID: 20976203, PMID: 28254838).

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	IF	Adv Sci (Weinh); cAMP-Induced Nuclear Condensation of CRTC2 Promotes Transcription Elongation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease.; Authors - Zeyun Mi
mouse	WB,IP	Circ Res; Positive transcription elongation factor b activity in compensatory myocardial hypertrophy is regulated by cardiac lineage protein-1.; Authors - Espinoza-Derout Jorge J
human	IHC	Acta Neuropathol; Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.; Authors - Neumann Manuela M
human	WB	Nucleic Acids Res; HMGA1 recruits CTIP2-repressed P-TEFb to the HIV-1 and cellular target promoters.; Authors - Sebastian Eilebrecht
human	WB	MBio; Interaction of the Ankyrin H Core Effector of Legionella with the Host LARP7 Component of the 7SK snRNP Complex.; Authors - Juanita Von Dwingelo

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