CoraLite® Plus 488-conjugated HELLS Polyclonal antibody

HELLS Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11955

Synonyms

Nbla10143, Lymphoid-specific helicase, Lymphoid specific helicase, LSH, helicase, lymphoid

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-11955

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 HELLS antibody (CL488-11955) at dilution of 1:200.

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  FC experiment of HepG2 using CL488-11955

  1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 HELLS Polyclonal Antibody (CL488-11955)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11955 targets HELLS in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HELLS fusion protein Ag2558
• Full Name: helicase, lymphoid-specific
• Calculated Molecular Weight: 348 aa, 40 kDa, 97 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC031004
• Gene Symbol: HELLS
• Gene ID (NCBI): 3070
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Lymphoid-specific helicase(HELLS) is a member of the SNF2 family of chromatin remodeling proteins that is required for development and survival. It's required for de novo or maintenance DNA methylation, which mediated the silencing of the imprinted CDKN1C gene. Also it involved in formation and organization of heterochromatin that regulate transcription and mitosis.