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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated HDAC2 Polyclonal antibody

HDAC2 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12922

Synonyms

YAF1, RPD3, histone deacetylase 2, HD2, EC:3.5.1.98

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated HDAC2 Polyclonal ant (CL488-12922)

    IF Staining of HepG2 using CL488-12922

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 HDAC2 antibody (CL488-12922) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HEK-293T cells using CoraLite® Plus 488-conjugated HDAC2 Polyclonal ant (CL488-12922)

    FC experiment of HEK-293T using CL488-12922

    1X10^6 HEK-293T cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human HDAC2 (CL488-12922) (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-12922 targets HDAC2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HDAC2 fusion protein Ag3607
Full Name histone deacetylase 2
Calculated Molecular Weight458 aa, 52 kDa; 488 aa,55 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC031055
Gene Symbol HDAC2
Gene ID (NCBI) 3066
RRIDAB_2919064
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human HDAC2.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 HDAC2 antibody CL488-12922Download protocol
FC protocol for CL Plus 488 HDAC2 antibody CL488-12922Download protocol
Standard Protocols
Click here to view our Standard Protocols