• Featured Product
  • KD/KO Validated

HDAC2 Polyclonal antibody

HDAC2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 12922-3-AP


HD2, HDAC2, histone deacetylase 2, RPD3, YAF1

Tested Applications

Positive WB detected inHEK-293 cells, human kidney tissue, MCF-7 cells, rat liver tissue, HeLa cells, HepG2 cells, L02 cells, C6 cells, NIH/3T3 cells, rat kidney tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman prostate cancer tissue, human breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHEK-293T cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:2000
Immunofluorescence (IF)IF : 1:200-1:2000
Sample-dependent, check data in validation data gallery

Product Information

12922-3-AP targets HDAC2 in WB, IP, IHC, IF, FC, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HDAC2 fusion protein Ag3607
Full Name histone deacetylase 2
Calculated molecular weight458 aa, 52 kDa; 488 aa,55 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC031055
Gene symbol HDAC2
Gene ID (NCBI) 3066
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human HDAC2.


Product Specific Protocols
WB protocol for HDAC2 antibody 12922-3-APDownload protocol
IHC protocol for HDAC2 antibody 12922-3-APDownload protocol
IF protocol for HDAC2 antibody 12922-3-APDownload protocol
IP protocol for HDAC2 antibody 12922-3-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Life Sci

Mucin 1 downregulation impairs the anti-necroptotic effects of glucocorticoids in human bronchial epithelial cells.

Authors - Huojun Zhang

J Cell Mol Med

LncRNA NKILA integrates RXFP1/AKT and NF-κB signalling to regulate osteogenesis of mesenchymal stem cells.

Authors - Ying Zhang

Clin Transl Med

EGFR-vIII downregulated H2AZK4/7AC though the PI3K/AKT-HDAC2 axis to regulate cell cycle progression.

Authors - Hongyu Zhao
  • KD Validated


Hepatic COX-2 expression protects mice from an alcohol-high fat diet-induced metabolic disorder by involving protein acetylation related energy metabolism.

Authors - Minjie Chen

Cell Death Discov

Epigenetic derepression converts PPARγ into a druggable target in triple-negative and endocrine-resistant breast cancers.

Authors - Ser Yue Loo

Acta Pharm Sin B

Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy.

Authors - Xin Sun


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Iram (Verified Customer) (09-17-2020)

HDAC2 antibody is very good. Giving very clear bands and very good staining in tissue sections

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:1000 for western and 1:100 for IHC
  • Cell Tissue Type: Human colon cancer

Dipen (Verified Customer) (09-04-2020)

Excellent antibody. One single clean band at the correct molecular weight.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Neuron
HDAC2 Antibody Western Blot validation (1:1000 dilution) in Neuron (Cat no:12922-3-AP)