- Featured Product
- KD/KO Validated
HDAC2 Polyclonal antibody
HDAC2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, chIP, ELISA
Conjugate
Unconjugated
Cat no : 12922-3-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, human kidney tissue, MCF-7 cells, rat liver tissue, HeLa cells, HepG2 cells, L02 cells, C6 cells, NIH/3T3 cells, rat kidney tissue |
Positive IP detected in | mouse testis tissue |
Positive IHC detected in | human prostate cancer tissue, human breast cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HEK-293T cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:200-1:2000 |
Immunofluorescence (IF) | IF : 1:200-1:2000 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 10 publications below |
WB | See 39 publications below |
IHC | See 10 publications below |
IF | See 5 publications below |
IP | See 4 publications below |
ChIP | See 1 publications below |
Product Information
12922-3-AP targets HDAC2 in WB, IP, IHC, IF, FC, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HDAC2 fusion protein Ag3607 |
Full Name | histone deacetylase 2 |
Calculated molecular weight | 458 aa, 52 kDa; 488 aa,55 kDa |
Observed molecular weight | 55-60 kDa |
GenBank accession number | BC031055 |
Gene symbol | HDAC2 |
Gene ID (NCBI) | 3066 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human HDAC2.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for HDAC2 antibody 12922-3-AP | Download protocol |
IHC protocol for HDAC2 antibody 12922-3-AP | Download protocol |
IF protocol for HDAC2 antibody 12922-3-AP | Download protocol |
IP protocol for HDAC2 antibody 12922-3-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Life Sci Mucin 1 downregulation impairs the anti-necroptotic effects of glucocorticoids in human bronchial epithelial cells. | ||
J Cell Mol Med LncRNA NKILA integrates RXFP1/AKT and NF-κB signalling to regulate osteogenesis of mesenchymal stem cells. | ||
Clin Transl Med EGFR-vIII downregulated H2AZK4/7AC though the PI3K/AKT-HDAC2 axis to regulate cell cycle progression.
| ||
Alcohol Hepatic COX-2 expression protects mice from an alcohol-high fat diet-induced metabolic disorder by involving protein acetylation related energy metabolism. | ||
Cell Death Discov Epigenetic derepression converts PPARγ into a druggable target in triple-negative and endocrine-resistant breast cancers. | ||
Acta Pharm Sin B Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Iram (Verified Customer) (09-17-2020) | HDAC2 antibody is very good. Giving very clear bands and very good staining in tissue sections
|
FH Dipen (Verified Customer) (09-04-2020) | Excellent antibody. One single clean band at the correct molecular weight.
![]() |