HDAC2 Polyclonal antibody

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  WB analysis using 12922-3-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12922-3-AP (HDAC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 12922-3-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 12922-3-AP (HDAC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12922-3-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12922-3-AP (HDAC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12922-3-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12922-3-AP (HDAC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 12922-3-AP

  IP result of anti-HDAC2 (IP:12922-3-AP, 4ug; Detection:12922-3-AP 1:20000) with HEK-293 cells lysate 960 ug.

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  IHC staining of mouse brain using 12922-3-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12922-3-AP (HDAC2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12922-3-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12922-3-AP (HDAC2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12922-3-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HDAC2 antibody (12922-3-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  FC experiment of HeLa using 12922-3-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HDAC2 Polyclonal antibody (12922-3-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• Featured Product
• KD/KO Validated

HDAC2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 12922-3-AP

Publications(92)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Synonyms

EC:3.5.1.-, EC:3.5.1.98, HD2, histone deacetylase 2, RPD3

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Tested Applications

• Positive WB detected in: HEK-293 cells, human kidney tissue, MCF-7 cells, rat liver tissue, HeLa cells, HepG2 cells, L02 cells, C6 cells, NIH/3T3 cells, rat kidney tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 14 publications below
• WB: See 80 publications below
• IHC: See 14 publications below
• IF: See 9 publications below
• IP: See 8 publications below
• CoIP: See 4 publications below
• ChIP: See 4 publications below

Product Information

12922-3-AP targets HDAC2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HDAC2 fusion protein Ag3607
• Full Name: histone deacetylase 2
• Calculated Molecular Weight: 458 aa, 52 kDa; 488 aa,55 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC031055
• Gene Symbol: HDAC2
• Gene ID (NCBI): 3066
• RRID: AB_2118516
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92769
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human HDAC2.

Publications

Species	Application	Title
rat	IP	Cell Metab; Sphingolipid metabolism controls mammalian heart regeneration; Authors - Xiaoqian Ji
human	CoIP	J Clin Invest; SAP30 promotes breast tumor progression by bridging the transcriptional corepressor SIN3 complex and MLL1; Authors - Lei Bao
human	WB	Acta Pharm Sin B; Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy.; Authors - Xin Sun
mouse,human	IHC,IF	Acta Pharm Sin B; PIM1-HDAC2 axis modulates intestinal homeostasis through epigenetic modification; Authors - Jianming Yang
mouse	WB	J Clin Invest; Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.; Authors - Shanshan Zhong
mouse	IP	Nat Commun; The methyltransferase METTL3 negatively regulates nonalcoholic steatohepatitis (NASH) progression.; Authors - Xinzhi Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zhongwen (Verified Customer) (09-25-2023)

There are two bands close to each other. Maybe they are different isoforms. But the quality is quite good.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

HDAC2 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:12922-3-AP)

FH

Iram (Verified Customer) (09-17-2020)

HDAC2 antibody is very good. Giving very clear bands and very good staining in tissue sections

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000 for western and 1:100 for IHC
• Cell Tissue Type: Human colon cancer

FH

Dipen (Verified Customer) (09-04-2020)

Excellent antibody. One single clean band at the correct molecular weight.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Neuron

HDAC2 Antibody Western Blot validation (1:1000 dilution) in Neuron (Cat no:12922-3-AP)