• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

HRP-conjugated HA Tag Monoclonal antibody

HA Tag Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein

Applications

WB

Conjugate

HRP

CloneNo.

1F5C6

Cat no : HRP-66006

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Recombinant protein using HRP-conjugated HA Tag Monoclonal antibody (HRP-66006)

    WB analysis of Recombinant protein using HRP-66006

    Recombinant protein and HEK-293 lysate were subjected to SDS PAGE followed by western blot with HRP-66006 (HA Tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

HRP-66006 targets HA Tag in WB applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name HA Tag
Gene Symbol
Gene ID (NCBI)
Conjugate HRP
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.

Publications

SpeciesApplicationTitle
WB

J Cancer

KRT80 Promotes Lung Adenocarcinoma Progression and Serves as a Substrate for VCP

Authors - Shanhua Huang
View Article