HA Tag Monoclonal antibody

HA Tag Monoclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein and More (6)

Applications

WB,IP,IF,FC,ELISA

Conjugate

Unconjugated

Publications

177

CloneNo.

1F5C6

Reviews (1)

Cat no : 66006-2-Ig

Synonyms

HA Tag, YPYDVPDYA

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• Validation Data Gallery
• Proteintech vs. Other Vendors

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  WB analysis of HEK-293 using 66006-2-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66006-2-Ig (HA Tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of Ag19172 using 66006-2-Ig

  HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.1μg/mL.

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  WB analysis of Ag19172 using 66006-2-Ig

  HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.05μg/mL.

• 

  WB analysis of Ag19172 using 66006-2-Ig

  HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.025μg/mL.

• 

  WB analysis of Ag19172 using 66006-2-Ig

  HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.0125μg/mL.

• 

  IP experiment of Transfected HEK-293 using 66006-2-Ig

  IP result of anti-HA Tag (IP:66006-2-Ig, 5ug; Detection:66006-2-Ig 1:2000) with Transfected HEK-293 cells lysate 1600 ug.

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  IF Staining of Transfected HEK-293 using 66006-2-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using HA Tag antibody (66006-2-Ig, Clone: 1F5C6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of Transfected HEK-293 using 66006-2-Ig

  1X10^6 Transfected HEK-293 cells were stained with 0.2 ug Anti- HA Tag (66006-2-Ig, Clone:1F5C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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  FC experiment of Transfected HEK-293 using 66006-2-Ig

  1X10^6 Transfected HEK-293 cells were stained with 0.2 ug Anti-N/A HA Tag (66006-2-Ig, Clone:1F5C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

• 

  FC experiment of Transfected HEK-293 using 66006-2-Ig

  1X10^6 Transfected HEK-293 cells were stained with 0.2 ug Anti-N/A HA Tag (66006-2-Ig, Clone:1F5C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: Transfected HEK-293 cells, HEK-293 cells, Ag19172
• Positive IP detected in: Transfected HEK-293 cells
• Positive IF detected in: Transfected HEK-293 cells
• Positive FC detected in: Transfected HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:10000-1:100000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:400-1:1600
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 122 publications below
• IF: See 21 publications below
• IP: See 39 publications below
• ELISA: See 1 publications below
• CoIP: See 21 publications below
• ChIP: See 1 publications below

Product Information

66006-2-Ig targets HA Tag in WB,IP,IF,FC,ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Cited Reactivity: human, mouse, rat, yeast, pig, canine
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: HA Tag
• RRID: AB_2881490
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.

Publications

Species	Application	Title
	WB	Cell; SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9; Authors - Nora Schmidt
		Cell Res; Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.; Authors - Fei Peng
human	IP	Nat Commun; Targeting the transcription factor HES1 by L-menthol restores protein phosphatase 6 in keratinocytes in models of psoriasis; Authors - Zhikai Wang
	IP	J Clin Invest; FAM117B promotes gastric cancer growth and drug resistance by targeting the KEAP1/NRF2 signaling pathway; Authors - Yunjiang Zhou
	WB	Nat Microbiol; IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection.; Authors - Zhimin Jiang
	WB	Acta Pharm Sin B; Nuciferine protects against high-fat diet-induced hepatic steatosis and insulin resistance via activating TFEB-mediated autophagy-lysosomal pathway.; Authors - Xiliang Du

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Parijat (Verified Customer) (03-06-2024)

Good antibody for immunofluorescence. Low background.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: 293T Cells