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CoraLite®555-conjugated HA Tag Monoclonal antibody

HA Tag Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein

Applications

IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1F5C6

Cat no : CL555-66006

Synonyms

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using CoraLite®555-conjugated HA Tag Monoclonal antibody (CL555-66006)

    IF Staining of Transfected HEK-293 using CL555-66006

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite®555 HA Tag antibody (CL555-66006, Clone: 1F5C6 ) at dilution of 1:600.

Tested Applications

Positive IF/ICC detected inTransfected HEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66006 targets HA Tag in IF/ICC applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name HA Tag
Gene Symbol
Gene ID (NCBI)
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.

Protocols

Product Specific Protocols
IF protocol for CL555 HA Tag antibody CL555-66006Download protocol
Standard Protocols
Click here to view our Standard Protocols