Glucagon Monoclonal antibody

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  IHC staining of human pancreas using 67286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 67286-1-Ig (Glucagon antibody) at dilution of 1:10000(under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 67286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 67286-1-Ig (Glucagon antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 67286-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67286-1-Ig (Glucagon antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 67286-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67286-1-Ig (Glucagon antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 67286-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67286-1-Ig (Glucagon antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human pancreas using 67286-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 67286-1-Ig (Glucagon antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). The B cells of the islets of Langerhans was labelled in red with 15848-1-AP (Insulin antibody).

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  IF Staining of mouse pancreas using 67286-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Glucagon antibody (67286-1-Ig, Clone: 2A8G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse pancreas using 67286-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Glucagon antibody (67286-1-Ig, Clone: 2A8G1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), INS antibody (15848-1-AP, red).

Glucagon Monoclonal Antibody for IHC, IF-P, ELISA

Cat No. 67286-1-Ig

Clone No.2A8G1

Publications(9)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (1)

Applications

IHC, IF-P, ELISA

Synonyms

GCG, GLP 2, GLP1, GLP2, glucagon, GRPP, GRPP Oxyntomodulin, OXM

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Tested Applications

• Positive IHC detected in: human pancreas tissue, mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse pancreas tissue, human pancreas tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:2000-1:15000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 7 publications below

Product Information

67286-1-Ig targets Glucagon in IHC, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Glucagon fusion protein Ag10629
• Full Name: glucagon
• Calculated Molecular Weight: 180 aa, 21 kDa
• GenBank Accession Number: BC005278
• Gene Symbol: Glucagon
• Gene ID (NCBI): 2641
• RRID: AB_2882552
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P01275
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glucagon is a 29-amino acid peptide hormone secreted from the pancreatic alpha cells with a powerful stimulatory effect on hepatic glucose production acting to increase plasma glucose levels. Glucagon is best known as the counter-regulatory hormone to INS, and normal glucose homeostasis depends largely on the balanced secretion of INS and glucagon from the pancreatic beta and alpha cells, respectively.The regulation of glucose metabolism by glucagon is mediated by its direct action on the peripheral tissues such as the liver and also by the brain. Glucagon is also released postprandially in a transient manner, which was shown to be involved in inhibition of food intake via reduction of meal size.

Publications

Species	Application	Title
mouse	IF	Food Chem X; Dendrobium officinale leaf polysaccharides ameliorated hyperglycemia and promoted gut bacterial associated SCFAs to alleviate type 2 diabetes in adult mice.; Authors - Jingyu Fang
mouse	IF	Life Sci Alliance; ciBAR1 loss in mice causes laterality defects, pancreatic degeneration, and altered glucose tolerance; Authors - Eunice N Kim
mouse	IF	Mol Cell Endocrinol; Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells.; Authors - Xinghong Guo
mouse	IHC	Life Sci; Semaglutide alleviates the pancreatic β cell function via the METTL14 signaling and modulating gut microbiota in type 2 diabetes mellitus mice; Authors - Yunfei Luo
mouse	IF	NPJ Biofilms Microbiomes; Bacteroides ovatus accelerates metformin-induced vitamin B12 deficiency in type 2 diabetes patients by accumulating cobalamin; Authors - Manyun Chen
human		Mol Metab; Long non-coding RNA LncCplx2 regulates glucose homeostasis and pancreatic β cell function; Authors - Linlin Wang