INS Polyclonal antibody
INS Polyclonal Antibody for IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 15848-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | human pancreas tissue, mouse pancreas tissue, rat pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human pancreas tissue, mouse pancreas tissue, rat pancreas tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 34 publications below |
| IF | See 30 publications below |
| ELISA | See 1 publications below |
Product Information
15848-1-AP targets INS in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | INS fusion protein Ag8630 |
| Full Name | INS |
| Calculated Molecular Weight | 110 aa, 12 kDa |
| GenBank Accession Number | BC005255 |
| Gene Symbol | INS |
| Gene ID (NCBI) | 3630 |
| RRID | AB_10597100 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
INS is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. INS is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form INS, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in INS results in type 1 diabetes mellitus.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for INS antibody 15848-1-AP | Download protocol |
| IF protocol for INS antibody 15848-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Protoc Isolation of mouse pancreatic islet Procr+ progenitors and long-term expansion of islet organoids in vitro. | ||
Autophagy Secretory autophagy promotes RAB37-mediated insulin secretion under glucose stimulation both in vitro and in vivo | ||
Theranostics Circular RNA circPPM1F modulates M1 macrophage activation and pancreatic islet inflammation in type 1 diabetes mellitus. | ||
ACS Appl Mater Interfaces Localized Controlled Release of Bilirubin from β-Cyclodextrin-Conjugated ε-Polylysine To Attenuate Oxidative Stress and Inflammation in Transplanted Islets. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Boyan (Verified Customer) (05-14-2021) | very good for IF
|
FH Isha (Verified Customer) (01-06-2021) | Worked really well with mice pancreas. Selectively stained islets beta cells, no background staining
|
