GSR Recombinant antibody

• 

  WB analysis using 84480-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84480-2-RR (GSR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 84480-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSR antibody (84480-2-RR, Clone: 241934D2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 84480-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug GSR Recombinant antibody (84480-2-RR, Clone:241934D2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 84480-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84480-2-RR against Human GSR were performed. The affinity constant is below 1 pM.

GSR {ptg:Brand} Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 84480-2-RR

Clone No.241934D2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

GRD1, GRase, glutathione reductase, GLUR, EC:1.8.1.7

---

Tested Applications

• Positive WB detected in: A549 cells, Jurkat cells, HeLa cells, mouse testis tissue
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84480-2-RR targets GSR in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GSR fusion protein Ag13080
• Full Name: glutathione reductase
• Calculated Molecular Weight: 522 aa, 56 kDa
• Observed Molecular Weight: 52-55 kDa
• GenBank Accession Number: BC069244
• Gene Symbol: GSR
• Gene ID (NCBI): 2936
• RRID: AB_3672000
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P00390
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSR(Glutathione reductase) is also named as GLUR, GRD1 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.It was first found in a black American a variant red cell GSR characterized by greater electrophoretic mobility and enzyme activity per unit of hemoglobin than the normal.This protein maintains high levels of reduced glutathione in the cytosol.It has 5 isoforms produced by alternative splicing and alternative initiation.