GSR Recombinant antibody, PBS Only (Capture)

GSR Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241934D2

Cat no : 84480-2-PBS

Synonyms

GRD1, GRase, glutathione reductase, GLUR, EC:1.8.1.7

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Validation Data Gallery

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  WB analysis using 84480-2-RR (same clone as 84480-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84480-2-RR (GSR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84480-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84480-2-RR (same clone as 84480-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSR antibody (84480-2-RR, Clone: 241934D2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84480-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01316-1

  Cytometric bead array standard curve of MP01316-1, GSR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84480-2-PBS. Detection antibody: 84480-1-PBS. Standard: Ag13080. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP01316-2

  Cytometric bead array standard curve of MP01316-2, GSR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84480-2-PBS. Detection antibody: 84480-3-PBS. Standard: Ag13080. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84480-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84480-2-RR against Human GSR were performed. The affinity constant is below 1 pM.

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Product Information

84480-2-PBS targets GSR as part of a matched antibody pair:

MP01316-1: 84480-2-PBS capture and 84480-1-PBS detection (validated in Cytometric bead array)

MP01316-2: 84480-2-PBS capture and 84480-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GSR fusion protein Ag13080
• Full Name: glutathione reductase
• Calculated Molecular Weight: 522 aa, 56 kDa
• Observed Molecular Weight: 52-55 kDa
• GenBank Accession Number: BC069244
• Gene Symbol: GSR
• Gene ID (NCBI): 2936
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GSR(Glutathione reductase) is also named as GLUR, GRD1 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.It was first found in a black American a variant red cell GSR characterized by greater electrophoretic mobility and enzyme activity per unit of hemoglobin than the normal.This protein maintains high levels of reduced glutathione in the cytosol.It has 5 isoforms produced by alternative splicing and alternative initiation.