CoraLite®594-conjugated GFP tag Monoclonal antibody

GFP tag Monoclonal Antibody for WB, IF, FC (Intra)

Host / Isotype

Mouse / IgG2a


recombinant protein


WB, IF, FC (Intra)


CoraLite®594 Fluorescent Dye



Cat no : CL594-66002


CFP, eGFP, eYFP, GFP, GFP tag, GFP-tag, green, green fluorescence protein, green fluorescent protein, YFP

Tested Applications

Positive WB detected inRecombinant protein
Positive IF detected inTransfected HEK-293 cells
Positive FC detected inTransfected HEK-293 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66002 targets GFP tag in WB, IF, FC (Intra) applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GFP tag fusion protein Ag2128
Full Name GFP tag
Calculated Molecular Weight 26 kDa
GenBank Accession NumberU73901
Gene Symbol
Gene ID (NCBI)
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. 

Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.

When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a mouse (IgG2a) monoclonal antibody against GFP reactive to GFP, eGFP, eYFP, YFP and CFP. This antibody is labeled with CoraLite Plus 594.


Product Specific Protocols
WB protocol for CL594 GFP tag antibody CL594-66002Download protocol
IF protocol for CL594 GFP tag antibody CL594-66002Download protocol
Standard Protocols
Click here to view our Standard Protocols