CoraLite® Plus 488-conjugated GFP tag Monoclonal antibody

GFP tag Monoclonal Antibody for WB, IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

recombinant protein

Applications

WB, IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E10H7

Cat no : CL488-66002

Synonyms

eGFP, GFP, green, GFP-tag, eYFP

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Validation Data Gallery

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  WB analysis using CL488-66002

  Recombinant protein were subjected to SDS PAGE followed by western blot with CL488-66002 (GFP tag antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of Transfected HEK-293 using CL488-66002

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 488 GFP tag antibody (CL488-66002, Clone: 1E10H7 ) at dilution of 1:200.

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Tested Applications

• Positive WB detected in: Recombinant protein
• Positive IF/ICC detected in: Transfected HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66002 targets GFP tag in WB, IF/ICC applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: GFP tag fusion protein Ag2128
• Full Name: GFP tag
• Calculated Molecular Weight: 26 kDa
• GenBank Accession Number: M62653
• RRID: AB_2919276
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. 

Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.

When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a mouse (IgG2a) monoclonal antibody against GFP reactive to GFP, eGFP, eYFP, YFP and CFP. This antibody is labeled with CoraLite Plus 488.