Biotin-conjugated GFAP Monoclonal antibody

GFAP Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

IHC

Conjugate

Biotin

CloneNo.

4B2E10

Cat no : Biotin-60190

Synonyms

FLJ45472, GFAP

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Validation Data Gallery

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  IHC staining of rat brain using Biotin-60190

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using Biotin-60190 (GFAP antibody) at dilution of 1:15000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using Biotin-60190

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using Biotin-60190 (GFAP antibody) at dilution of 1:15000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:7500-1:30000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-60190 targets GFAP in IHC applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: GFAP fusion protein Ag10452
• Full Name: glial fibrillary acidic protein
• Calculated Molecular Weight: 432 aa, 50 kDa
• Observed Molecular Weight: 45-52 kDa
• GenBank Accession Number: BC013596
• Gene Symbol: GFAP
• Gene ID (NCBI): 2670
• RRID: AB_2918946
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Function

GFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central nervous system (CNS). GFAP is one of the main components of the intermediate filament network in astrocytes and has been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis.

Tissue specificity

GFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker. However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes, and liver stellate cells (PMID: 21219963).

Involvement in disease

• Mutations in GFAP lead to Alexander disease (OMIM: 203450), an autosomal dominant CNS disorder. The mutations present in affected individuals are thought to be gain-of-function.
• Upregulation of GFAP is a hallmark of reactive astrocytes, in which GFAP is present in hypertrophic cellular processes. Reactive astrogliosis is present in many neurological disorders, such as stroke, various neurodegenerative diseases (including Alzheimer's and Parkinson's disease), and neurotrauma.

Isoforms

Astrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916), which means that they differ in molecular size. Isoform expression varies during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes.

Post-translational modifications

Intermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP protein, at least some of which are reported to control filament assembly (PMID: 21219963).

Cellular localization

GFAP localizes to intermediate filaments and stains well in astrocyte cellular processes.