GFAP Monoclonal antibody, PBS Only

GFAP Monoclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

CloneNo.

4B2E10

Cat no : 60190-1-PBS

Synonyms

glial fibrillary acidic protein, 4B2E10

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Validation Data Gallery

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  WB analysis using 60190-1-Ig (same clone as 60190-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60190-1-Ig (GFAP antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

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  WB analysis of U-251 using 60190-1-Ig (same clone as 60190-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 60190-1-Ig (GFAP Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 60190-1-Ig (same clone as 60190-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 60190-1-Ig (GFAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 60190-1-Ig (same clone as 60190-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60190-1-Ig (GFAP antibody) at dilution of 1:300000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 60190-1-Ig (same clone as 60190-1-PBS)

  IP result of anti-GFAP (IP:60190-1-Ig, 5ug; Detection:60190-1-Ig 1:1000) with mouse brain tissue lysate 2640ug. This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 60190-1-Ig (same clone as 60190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60190-1-Ig (GFAP Antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0. This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 60190-1-Ig (same clone as 60190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60190-1-Ig (GFAP antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 60190-1-Ig (same clone as 60190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60190-1-Ig (GFAP antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 60190-1-Ig (same clone as 60190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60190-1-Ig (GFAP Antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0. This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 60190-1-Ig (same clone as 60190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60190-1-Ig (GFAP Antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 60190-1-Ig (same clone as 60190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60190-1-Ig (GFAP Antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 60190-1-Ig (same clone as 60190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60190-1-Ig (GFAP antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 60190-1-Ig (same clone as 60190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60190-1-Ig (GFAP antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 60190-1-Ig (same clone as 60190-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using GFAP antibody (60190-1-Ig, Clone: 4B2E10 ) at dilution of 1:200 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 60190-1-Ig (same clone as 60190-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using GFAP antibody (60190-1-Ig, Clone: 4B2E10 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

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Product Information

60190-1-PBS targets GFAP in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: GFAP fusion protein Ag10452
• Full Name: glial fibrillary acidic protein
• Calculated Molecular Weight: 432 aa, 50 kDa
• Observed Molecular Weight: 45-52 kDa
• GenBank Accession Number: BC013596
• Gene Symbol: GFAP
• Gene ID (NCBI): 2670
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Function

GFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central nervous system (CNS). GFAP is one of the main components of the intermediate filament network in astrocytes and has been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis.

Tissue specificity

GFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker. However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes, and liver stellate cells (PMID: 21219963).

Involvement in disease

• Mutations in GFAP lead to Alexander disease (OMIM: 203450), an autosomal dominant CNS disorder. The mutations present in affected individuals are thought to be gain-of-function.
• Upregulation of GFAP is a hallmark of reactive astrocytes, in which GFAP is present in hypertrophic cellular processes. Reactive astrogliosis is present in many neurological disorders, such as stroke, various neurodegenerative diseases (including Alzheimer's and Parkinson's disease), and neurotrauma.

Isoforms

Astrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916), which means that they differ in molecular size. Isoform expression varies during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes.

Post-translational modifications

Intermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP protein, at least some of which are reported to control filament assembly (PMID: 21219963).

Cellular localization

GFAP localizes to intermediate filaments and stains well in astrocyte cellular processes.