GBP2 Recombinant antibody, PBS Only

GBP2 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241580C12

Cat no : 84264-6-PBS

Synonyms

Guanine nucleotide-binding protein 2, GTP-binding protein 2, GBP-2, GBP 2, EC:3.6.5.-

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Validation Data Gallery

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  WB analysis using 84264-6-RR (same clone as 84264-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84264-6-RR (GBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84264-6-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84264-6-RR (same clone as 84264-6-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GBP2 antibody (84264-6-RR, Clone: 241580C12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84264-6-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 84264-6-RR (same clone as 84264-6-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.2 ug GBP2 Recombinant antibody (84264-6-RR, Clone:241580C12) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84264-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84264-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84264-6-RR against Human GBP2 were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 84264-6-PBS is GBP2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GBP2 fusion protein Ag2425
• Full Name: guanylate binding protein 2, interferon-inducible
• Calculated Molecular Weight: 591 aa, 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC022272
• Gene Symbol: GBP2
• Gene ID (NCBI): 2634
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GBP2 is a member of the GTPase family and is crucial to host immunity against pathogens. GBP2 is also used as a marker of interferon (IFN) responsiveness because it is one of the most highly expressed genes after IFN-γ stimulation. Increased GBP2 expression is also associated with a better prognosis in breast cancer and may have a role in T-cell defense against breast cancer. Interestingly, GBP2 expression in NIH/3T3 fibroblasts inhibited Rac activation and matrix metalloproteinase-9 expression, suggesting a possible role for GBP2 in regulating cancer metastasis. The molecular weight of GBP2 is 67 kDa. (PMID: 29072687, 35383115)