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GBP2 Recombinant antibody

GBP2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241580C12

Cat no : 84264-6-RR

Synonyms

Guanine nucleotide-binding protein 2, GTP-binding protein 2, GBP-2, GBP 2, EC:3.6.5.-

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GBP2 Recombinant antibody (84264-6-RR)

    WB analysis using 84264-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84264-6-RR (GBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GBP2 Recombinant antibody (84264-6-RR)

    IF Staining of HeLa using 84264-6-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GBP2 antibody (84264-6-RR, Clone: 241580C12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of A549 cells using GBP2 Recombinant antibody (84264-6-RR)

    FC experiment of A549 using 84264-6-RR

    1x10^6 A549 cells were intracellularly stained with 0.2 ug GBP2 Recombinant antibody (84264-6-RR, Clone:241580C12) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA549 cells, IM-9 cells, HaCaT cells, K-562 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84264-6-RR is GBP2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GBP2 fusion protein Ag2425
Full Name guanylate binding protein 2, interferon-inducible
Calculated Molecular Weight 591 aa, 67 kDa
Observed Molecular Weight67 kDa
GenBank Accession NumberBC022272
Gene Symbol GBP2
Gene ID (NCBI) 2634
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GBP2 is a member of the GTPase family and is crucial to host immunity against pathogens. GBP2 is also used as a marker of interferon (IFN) responsiveness because it is one of the most highly expressed genes after IFN-γ stimulation. Increased GBP2 expression is also associated with a better prognosis in breast cancer and may have a role in T-cell defense against breast cancer. Interestingly, GBP2 expression in NIH/3T3 fibroblasts inhibited Rac activation and matrix metalloproteinase-9 expression, suggesting a possible role for GBP2 in regulating cancer metastasis. The molecular weight of GBP2 is 67 kDa. (PMID: 29072687, 35383115)

Protocols

Product Specific Protocols
WB protocol for GBP2 antibody 84264-6-RRDownload protocol
IF protocol for GBP2 antibody 84264-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols