GATA6 Recombinant antibody, PBS Only

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  WB analysis using 84987-3-RR (same clone as 84987-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84987-3-RR (GATA6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84987-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84987-3-RR (same clone as 84987-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GATA6 antibody (84987-3-RR, Clone: 242325B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84987-3-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 84987-3-RR (same clone as 84987-3-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug GATA6 Recombinant antibody (84987-3-RR, Clone:242325B6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84987-3-PBS in a different storage buffer formulation.

GATA6 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 84987-3-PBS

Clone No.242325B6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Transcription factor GATA-6, Transcription factor GATA 6, GATA-binding factor 6, 242325B6

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Product Information

84987-3-PBS targets GATA6 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: GATA binding protein 6
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60-64 kDa
• GenBank Accession Number: NM_005257
• Gene Symbol: GATA6
• Gene ID (NCBI): 2627
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q92908
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

GATA6 is a member of the GATA family of zinc-finger transcriptional regulators, which play crucial roles in the regulation of cell growth, differentiation, survival, and maintenance of body functions. Named after the conserved base sequence (G/A)GATA(A/T), GATA6 features conserved tandem zinc fingers and is essential in coordinating the development and precise gene regulation of diverse tissues, including the heart and gastrointestinal tract. GATA6 recognizes the sequence (A/T/C)GATA(A/T)A and interacts with other transcriptional regulators through its zinc-finger domain.