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  • Western Blot (WB) analysis of various lysates using GATA6 Recombinant antibody (84987-3-RR)

    WB analysis using 84987-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84987-3-RR (GATA6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GATA6 Recombinant antibody (84987-3-RR)

    IF Staining of HeLa using 84987-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GATA6 antibody (84987-3-RR, Clone: 242325B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HEK-293 cells using GATA6 Recombinant antibody (84987-3-RR)

    FC experiment of HEK-293 using 84987-3-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug GATA6 Recombinant antibody (84987-3-RR, Clone:242325B6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

GATA6 Recombinant antibody

GATA6 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84987-3-RR
Clone No.242325B6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

Transcription factor GATA-6, Transcription factor GATA 6, GATA-binding factor 6, 242325B6

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells, MCF-7 cells, HepG2 cells, SGC-7901 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84987-3-RR targets GATA6 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide Predict reactive species
Full Name GATA binding protein 6
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60-64 kDa
GenBank Accession NumberNM_005257
Gene Symbol GATA6
Gene ID (NCBI) 2627
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ92908
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GATA6 is a member of the GATA family of zinc-finger transcriptional regulators, which play crucial roles in the regulation of cell growth, differentiation, survival, and maintenance of body functions. Named after the conserved base sequence (G/A)GATA(A/T), GATA6 features conserved tandem zinc fingers and is essential in coordinating the development and precise gene regulation of diverse tissues, including the heart and gastrointestinal tract. GATA6 recognizes the sequence (A/T/C)GATA(A/T)A and interacts with other transcriptional regulators through its zinc-finger domain.

Protocols

Product Specific Protocols
WB protocol for GATA6 antibody 84987-3-RRDownload protocol
IF protocol for GATA6 antibody 84987-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols