CoraLite® Plus 488-conjugated GAPDH Monoclonal antibody

GAPDH Monoclonal Antibody for FC (Intra), WB

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, zebrafish, yeast, plant

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E6D9

Cat no : CL488-60004

Synonyms

G3PD, GAPD, GAPDH, OK/SW cl.12



Tested Applications

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, Jurkat cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 7 publications below

Product Information

CL488-60004 targets GAPDH in WB, FC (Intra) applications and shows reactivity with human, mouse, rat, zebrafish, yeast, plant samples.

Tested Reactivity human, mouse, rat, zebrafish, yeast, plant
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GAPDH fusion protein Ag0766
Full Name glyceraldehyde-3-phosphate dehydrogenase
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC004109
Gene Symbol GAPDH
Gene ID (NCBI) 2597
RRIDAB_2919223
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 GAPDH antibody CL488-60004Download protocol
FC protocol for CL Plus 488 GAPDH antibody CL488-60004Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

Coagulation factor 2 thrombin receptor promotes malignancy in glioma under SOX2 regulation.

Authors - Guojun Gao
humanWB

Cell Commun Signal

Integral membrane protein 2A inhibits cell growth in human breast cancer via enhancing autophagy induction.

Authors - Cefan Zhou
humanWB

Sci Rep

Comparative analysis of pathophysiological parameters between emphysematous smokers and emphysematous patients with COPD.

Authors - Shuang Bai
humanWB

Exp Cell Res

CLEC12B suppresses lung cancer progression by inducing SHP-1 expression and inactivating the PI3K/AKT signaling pathway.

Authors - Decai Chi
ratWB

Virus Res

Interaction of porcine reproductive and respiratory syndrome virus major envelope proteins GP5 and M with the cellular protein Snapin.

Authors - Julie A Hicks
mouseWB

Reprod Fertil Dev

Effects of 2,3',4,4',5-pentachlorobiphenyl exposure during pregnancy on DNA methylation in the testis of offspring in the mouse.

Authors - Jian-Mei Zhang