CoraLite® Plus 488-conjugated GAPDH Monoclonal antibody

• Featured Product
• KD/KO Validated

GAPDH Monoclonal Antibody for WB, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, zebrafish, yeast, plant

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

8

CloneNo.

1E6D9

Cat no : CL488-60004

Synonyms

glyceraldehyde 3 phosphate dehydrogenase, GAPD, EC:2.6.99.-, EC:1.2.1.12, CDABP0047

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Validation Data Gallery

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  WB analysis using CL488-60004

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-60004 (GAPDH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using CL488-60004

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-60004 (GAPDH antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HeLa using CL488-60004

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GAPDH (CL488-60004, Clone:1E6D9) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, HEK-293 cells, Jurkat cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below

Product Information

CL488-60004 targets GAPDH in WB, FC (Intra) applications and shows reactivity with human, mouse, rat, zebrafish, yeast, plant samples.

• Tested Reactivity: human, mouse, rat, zebrafish, yeast, plant
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: GAPDH fusion protein Ag0766
• Full Name: glyceraldehyde-3-phosphate dehydrogenase
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC004109
• Gene Symbol: GAPDH
• Gene ID (NCBI): 2597
• RRID: AB_2919223
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Publications

Species	Application	Title
human	WB	Aging (Albany NY); Coagulation factor 2 thrombin receptor promotes malignancy in glioma under SOX2 regulation.; Authors - Guojun Gao
human	WB	Cell Commun Signal; Integral membrane protein 2A inhibits cell growth in human breast cancer via enhancing autophagy induction.; Authors - Cefan Zhou
human	WB	Sci Rep; Comparative analysis of pathophysiological parameters between emphysematous smokers and emphysematous patients with COPD.; Authors - Shuang Bai
human	WB	Exp Cell Res; CLEC12B suppresses lung cancer progression by inducing SHP-1 expression and inactivating the PI3K/AKT signaling pathway.; Authors - Decai Chi
rat	WB	Virus Res; Interaction of porcine reproductive and respiratory syndrome virus major envelope proteins GP5 and M with the cellular protein Snapin.; Authors - Julie A Hicks
mouse	WB	Reprod Fertil Dev; Effects of 2,3',4,4',5-pentachlorobiphenyl exposure during pregnancy on DNA methylation in the testis of offspring in the mouse.; Authors - Jian-Mei Zhang

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C