HRP-conjugated GAPDH Monoclonal antibody

GAPDH Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, zebrafish, plant and More (6)

Applications

WB, IP, IHC, IF

Conjugate

HRP

CloneNo.

1E6D9

Cat no : HRP-60004

Synonyms

G3PD, GAPD, GAPDH, HRP-G3PD, HRP-GAPD, HRP-GAPDH, HRP-OK/SW cl.12, OK/SW cl.12



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

HRP-60004 targets GAPDH in WB, IP, IHC, IF applications and shows reactivity with human, mouse, rat, zebrafish, plant samples.

Tested Reactivity human, mouse, rat, zebrafish, plant
Cited Reactivityhuman, mouse, rat, Chicken, Honey bees, rabbit, hamster, pig, opossum
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Recombinant Protein
Full Name glyceraldehyde-3-phosphate dehydrogenase
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC004109
Gene symbol GAPDH
Gene ID (NCBI) 2597
RRIDAB_2737588
Conjugate HRP
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Protocols

Product Specific Protocols
WB protocol for HRP GAPDH antibody HRP-60004Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25.

Authors - Linbin Yang
humanWB

Nat Biotechnol

Direct targeting of amplified gene loci for proapoptotic anticancer therapy.

Authors - Meetu Kaushik Tiwari
humanWB

Cell

Programmable RNA-Guided RNA Effector Proteins Built from Human Parts.

Authors - Simone Rauch
humanWB

Cell

Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages.

Authors - Shicheng Su
humanWB

Cell

CD10+GPR77+Cancer-Associated Fibroblasts Promote Cancer Formation and Chemoresistance by Sustaining Cancer Stemness.

Authors - Shicheng Su
human,mouseWB

Cell

PMID: 28753426

Authors - Kun Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tsimafei (Verified Customer) (07-15-2022)

30µg of total protein were loaded on the gel. Antibodies were incubated ON at 4C.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: mESCs
HRP GAPDH Antibody Western Blot validation (1:5000 dilution) in mESCs (Cat no:HRP-60004)
FH

Hala (Verified Customer) (02-25-2022)

it works very good but i got an aspecific band around 30KDa

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/15000
  • Cell Tissue Type: hepG2
HRP GAPDH Antibody Western Blot validation (1/15000 dilution) in hepG2 (Cat no:HRP-60004)
FH

Laura (Verified Customer) (01-10-2020)

Works well

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HEK293T
FH

Benjamin (Verified Customer) (01-07-2020)

Works really well with high sensitivity and removes the need for a secondary. Needs to be dilute as GAPDH bands will be too intense at higher dilutions.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:25000
  • Cell Tissue Type: HEK293T, SHSY5Y
FH

Kathryn (Verified Customer) (12-10-2019)

Used at 1:5000 in 5% BSA on a small amount of neonatal mouse ovary and worked beautifully.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: mouse ovary
HRP GAPDH Antibody Western Blot, validation (1:5000 dilution) in mouse ovary (Cat no:HRP-60004)