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- KD/KO Validated
HRP-conjugated GAPDH Monoclonal antibody
GAPDH Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, zebrafish, plant and More (7)
Applications
WB, IP, CoIP, RIP
Conjugate
HRP
669
CloneNo.
1E6D9
Cat no : HRP-60004
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 643 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
| RIP | See 1 publications below |
Product Information
HRP-60004 targets GAPDH in WB, IP, CoIP, RIP applications and shows reactivity with human, mouse, rat, zebrafish, plant samples.
| Tested Reactivity | human, mouse, rat, zebrafish, plant |
| Cited Reactivity | human, mouse, rat, pig, rabbit, canine, chicken, hamster, opossum, honey bees |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Recombinant Protein |
| Full Name | glyceraldehyde-3-phosphate dehydrogenase |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC004109 |
| Gene Symbol | GAPDH |
| Gene ID (NCBI) | 2597 |
| RRID | AB_2737588 |
| Conjugate | HRP |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP GAPDH antibody HRP-60004 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cancer Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer | ||
Nat Biotechnol Direct targeting of amplified gene loci for proapoptotic anticancer therapy. | ||
Cell Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages. | ||
Cell CD10+GPR77+Cancer-Associated Fibroblasts Promote Cancer Formation and Chemoresistance by Sustaining Cancer Stemness. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Moritz (Verified Customer) (09-12-2024) | The antibody worked very well at a dilution of 1:10k in WB using HEL293T cell lysate as a loading control.
 |
FH Julia (Verified Customer) (08-07-2024) | Really good antibody with specific band at the correct size. Conjugation with HRP allows quickworks great to get results quickly.
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FH T (Verified Customer) (03-20-2024) | Worked great with multiple tissue types. Highly recommend!
|
FH Tsimafei (Verified Customer) (07-15-2022) | 30µg of total protein were loaded on the gel. Antibodies were incubated ON at 4C.
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FH Hala (Verified Customer) (02-25-2022) | it works very good but i got an aspecific band around 30KDa
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FH Laura (Verified Customer) (01-10-2020) | Works well
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FH Benjamin (Verified Customer) (01-07-2020) | Works really well with high sensitivity and removes the need for a secondary. Needs to be dilute as GAPDH bands will be too intense at higher dilutions.
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FH Kathryn (Verified Customer) (12-10-2019) | Used at 1:5000 in 5% BSA on a small amount of neonatal mouse ovary and worked beautifully.
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