GAPDH Monoclonal antibody, PBS Only

GAPDH Monoclonal Antibody for WB, IP, IF, FC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, yeast, plant, zebrafish

Applications

WB, IP, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1E6D9

Cat no : 60004-1-PBS

Synonyms

G3PD, GAPD, GAPDH, OK/SW cl.12

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Validation Data Gallery

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  WB analysis using 60004-1-Ig (same clone as 60004-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60004-1-Ig (same clone as 60004-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60004-1-Ig (same clone as 60004-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 60004-1-Ig (same clone as 60004-1-PBS)

  Western blot analysis of GAPDH in various tissues and cell lines using Proteintech antibody 60004-1-Ig at a dilution of 1:10000. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

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  WB analysis of arabidopsis whole plant using 60004-1-Ig (same clone as 60004-1-PBS)

  arabidopsis, rice, wheat, corn whole plant tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60004-1-Ig (same clone as 60004-1-PBS)

  Western blot of Hela cell with anti-GAPDH (60004-1-Ig) at various dilutions. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

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  WB analysis of whole Nematode using 60004-1-Ig (same clone as 60004-1-PBS)

  whole Nematode tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

• 

  WB analysis of soybean whole plant using 60004-1-Ig (same clone as 60004-1-PBS)

  soybean whole plant tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

• 

  WB analysis of zebrafish using 60004-1-Ig (same clone as 60004-1-PBS)

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

• 

  WB analysis of whole yeast using 60004-1-Ig (same clone as 60004-1-PBS)

  whole yeast cells were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60004-1-Ig (same clone as 60004-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa Cells using 60004-1-Ig (same clone as 60004-1-PBS)

  IP result of anti-GAPDH (60004-1-Ig for IP and Detection) with HeLa cell lysate. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60004-1-Ig (same clone as 60004-1-PBS)

  Immunofluorescent analysis of EA treated HeLa cells using 60004-1-Ig(GAPDH antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 60004-1-Ig (same clone as 60004-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GAPDH (60004-1-Ig, Clone:1E6D9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60004-1-PBS targets GAPDH in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, yeast, plant, zebrafish samples.

• Tested Reactivity: human, mouse, rat, yeast, plant, zebrafish
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: GAPDH fusion protein Ag0766
• Full Name: glyceraldehyde-3-phosphate dehydrogenase
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC004109
• Gene Symbol: GAPDH
• Gene ID (NCBI): 2597
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable.