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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated G6PD Monoclonal antibody

G6PD Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2A7B12

Cat no : CL647-66373

Synonyms

G6P, G6PD, G6PD1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 647-conjugated G6PD Monoclonal anti (CL647-66373)

    FC experiment of HeLa using CL647-66373

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human G6PD (CL647-66373, Clone:2A7B12) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-66373 targets G6PD in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen G6PD fusion protein Ag21862
Full Name glucose-6-phosphate dehydrogenase
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC000337
Gene Symbol G6PD
Gene ID (NCBI) 2539
RRIDAB_2920281
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

G6PD(Glucose-6-phosphate 1-dehydrogenase) belongs to the glucose-6-phosphate dehydrogenase family. It is a housekeeping enzyme encoded in mammals by an X-linked gene. G6PD has important functions in intermediary metabolism because it catalyzes the first step in the pentose phosphate pathway and provides reductive potential in the form of NADPH.(PMID:7489710). This protein has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 G6PD antibody CL647-66373Download protocol
Standard Protocols
Click here to view our Standard Protocols