CoraLite® Plus 488-conjugated G6PD Monoclonal antibody

• Featured Product
• KD/KO Validated

G6PD Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

1

CloneNo.

2A7B12

Cat no : CL488-66373

Synonyms

Glucose-6-phosphate 1-dehydrogenase, G6PD1, G6P, EC:1.1.1.49, 2A7B12

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Validation Data Gallery

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  IF Staining of HeLa using CL488-66373

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 G6PD antibody (CL488-66373, Clone: 2A7B12 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-66373

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated G6PD Monoclonal antibody (CL488-66373, Clone:2A7B12)(red), or 0.4 ug Isotype Control (CL488-66360-2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66373 targets G6PD in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: G6PD fusion protein Ag21862
• Full Name: glucose-6-phosphate dehydrogenase
• Calculated Molecular Weight: 59 kDa
• GenBank Accession Number: BC000337
• Gene Symbol: G6PD
• Gene ID (NCBI): 2539
• RRID: AB_2883320
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

G6PD(Glucose-6-phosphate 1-dehydrogenase) belongs to the glucose-6-phosphate dehydrogenase family. It is a housekeeping enzyme encoded in mammals by an X-linked gene. G6PD has important functions in intermediary metabolism because it catalyzes the first step in the pentose phosphate pathway and provides reductive potential in the form of NADPH.(PMID:7489710). This protein has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
		Nat Commun; Alveolar macrophage metabolic programming via a C-type lectin receptor protects against lipo-toxicity and cell death; Authors - Michal Scur