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CoraLite®555-conjugated G6PD Monoclonal antibody
G6PD Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite®555 Fluorescent Dye
CloneNo.
2A7B12
Cat no : CL555-66373
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL555-66373 targets G6PD in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | G6PD fusion protein Ag21862 |
Full Name | glucose-6-phosphate dehydrogenase |
Calculated Molecular Weight | 59 kDa |
Observed Molecular Weight | 60 kDa |
GenBank Accession Number | BC000337 |
Gene Symbol | G6PD |
Gene ID (NCBI) | 2539 |
RRID | AB_2919684 |
Conjugate | CoraLite®555 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 557 nm / 570 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
Background Information
G6PD(Glucose-6-phosphate 1-dehydrogenase) belongs to the glucose-6-phosphate dehydrogenase family. It is a housekeeping enzyme encoded in mammals by an X-linked gene. G6PD has important functions in intermediary metabolism because it catalyzes the first step in the pentose phosphate pathway and provides reductive potential in the form of NADPH.(PMID:7489710). This protein has 3 isoforms produced by alternative splicing.
Protocols
Product Specific Protocols | |
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IF protocol for CL555 G6PD antibody CL555-66373 | Download protocol |
FC protocol for CL555 G6PD antibody CL555-66373 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |