CoraLite® Plus 647-conjugated DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)

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  IF Staining of Transfected HEK-293 using CL647-66008

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 647 DYKDDDDK tag antibody (CL647-66008, Clone: 8H6A10 ) at dilution of 1:200.

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  FC experiment of Transfected HEK-293T using CL647-66008

  1x10^6 Transfected HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) (CL647-66008, Clone:8H6A10). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

DYKDDDDK tag Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL647-66008

Clone No.8H6A10

Publications(1)

Reviews (1)

Host / Isotype

Mouse / IgG2b

Reactivity

recombinant protein

Applications

WB, IF/ICC, FC (Intra)

Synonyms

Flag, Flag tag, DDDDK, DDDDK tag, DYKDDDDK

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Tested Applications

• Positive IF/ICC detected in: Transfected HEK-293 cells
• Positive FC (Intra) detected in: Transfected HEK-293T cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

CL647-66008 targets DYKDDDDK tag in WB, IF/ICC, FC (Intra) applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: DYKDDDDK tag
• RRID: AB_2920272
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: FLAGTAG
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Adv Sci (Weinh); FUS Selectively Facilitates circRNAs Packing into Small Extracellular Vesicles within Hypoxia Neuron; Authors - Jiankun Zang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rushita (Verified Customer) (10-07-2025)

This antibody is a game-changer! Sensitive and spot on!

• Applications: Immunofluorescence
• Cell Tissue Type: Heart, Kindey, Liver