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CoraLite® Plus 647-conjugated DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)

DYKDDDDK tag Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

8H6A10

Cat no : CL647-66008

Synonyms

Flag, DYKDDDDK, DDDDK tag, DDDDK

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using CoraLite® Plus 647-conjugated DYKDDDDK tag Monoclo (CL647-66008)

    IF Staining of Transfected HEK-293 using CL647-66008

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 647 DYKDDDDK tag antibody (CL647-66008, Clone: 8H6A10 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using CoraLite® Plus 647-conjugated DYKDDDDK tag Monoclo (CL647-66008)

    FC experiment of Transfected HEK-293T using CL647-66008

    1x10^6 Transfected HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) (CL647-66008, Clone:8H6A10). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inTransfected HEK-293 cells
Positive FC (Intra) detected inTransfected HEK-293T cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

CL647-66008 targets DYKDDDDK tag in WB, IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name DYKDDDDK tag
Gene Symbol
Gene ID (NCBI)
RRIDAB_2920272
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 DYKDDDDK tag antibody CL647-66008Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Adv Sci (Weinh)

FUS Selectively Facilitates circRNAs Packing into Small Extracellular Vesicles within Hypoxia Neuron

Authors - Jiankun Zang
View Article