IF Staining of Transfected HEK-293 using CL647-66008
Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 647 DYKDDDDK tag antibody (CL647-66008, Clone: 8H6A10 ) at dilution of 1:200.
Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 647 DYKDDDDK tag antibody (CL647-66008, Clone: 8H6A10 ) at dilution of 1:200.
FC experiment of Transfected HEK-293T using CL647-66008
1x10^6 Transfected HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) (CL647-66008, Clone:8H6A10). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 Transfected HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) (CL647-66008, Clone:8H6A10). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
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IF Staining of Transfected HEK-293 using CL647-66008
Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 647 DYKDDDDK tag antibody (CL647-66008, Clone: 8H6A10 ) at dilution of 1:200.
FC (INTRA) Figures
FC experiment of Transfected HEK-293T using CL647-66008
1x10^6 Transfected HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) (CL647-66008, Clone:8H6A10). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
DYKDDDDK tag Monoclonal antibody
Catalog Number
CL647-66008
Citations
1
Dilutions
IF/ICC : 1:50-1:500 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
IF/ICC, FC (Intra)
Reactivity
recombinant protein
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
