HRP-conjugated DYKDDDDK Tag Monoclonal antibody (Binds to FLAG® tag epitope)

DYKDDDDK Tag Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2b

Reactivity

recombinant protein and More (2)

Applications

WB, CoIP, ELISA

Conjugate

HRP

Publications

19

CloneNo.

8H6A10

Cat no : HRP-66008

Synonyms

DDDDK tag, DYKDDDDK, DYKDDDDK Tag, HRP conjugated DDDDK tag, HRP-66008, HRP-DDDDK tag

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Validation Data Gallery

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  WB analysis of Recombinant protein using HRP-66008

  Recombinant protein were subjected to SDS PAGE followed by western blot with HRP-66008 (DYKDDDDK Tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:10000-1:100000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 14 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Product Information

HRP-66008 targets DYKDDDDK Tag in WB, CoIP, ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: DYKDDDDK Tag
• RRID: AB_2883835
• Conjugate: HRP
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DYKDDDDK tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDYKDDDDK. The structure of DYKDDDDK tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with DYKDDDDK tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 1E7B4, which is different from any other vendors. This antibody is HRP conjugated DYKDDDDK Tag antibody. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Publications

Species	Application	Title
	WB	Nat Commun; FAM222A encodes a protein which accumulates in plaques in Alzheimer's disease.; Authors - Tingxiang Yan
		PLoS Pathog; Vibrio splendidus infection promotes circRNA-FGL1-regulated coelomocyte apoptosis via competitive binding to Myc with the deubiquitinase OTUB1 in Apostichopus japonicus; Authors - Ming Guo
	WB	Int Immunopharmacol; ZAP facilitates NLRP3 inflammasome activation via promoting the oligomerization of NLRP3; Authors - Danhui Qin
	WB	Liver Int; The DEAD-box helicase DDX3x ameliorates non-alcoholic fatty liver disease via mTORC1 signalling pathway.; Authors - Peihao Liu
		Biomed Pharmacother; PMID: 32036219; Authors - Yu Chen
		Biochem Biophys Res Commun; Vaccination with a DNA vaccine encoding CD317-targeting HBs antigen elicits enhanced immunity in mice.; Authors - Guizhong Zhang