Biotin-conjugated DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)

DYKDDDDK tag Monoclonal Antibody for IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

recombinant protein

Applications

IF/ICC, ELISA

Conjugate

Biotin

CloneNo.

8H6A10

Cat no : Biotin-66008-4

Synonyms

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Validation Data Gallery

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  IF Staining of Transfected HEK-293 using Biotin-66008-4

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using Biotin DYKDDDDK tag antibody (Biotin-66008-4, Clone: 8H6A10 ) at dilution of 1:600 and Alexa Fluor 488 Streptavidin.

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  ELISA experiment of FusionProtein using Biotin-66008-4

  Ag2329 (DYKDDDDK tagged protein) was coated onto microtiter plates at 0.073 µg/well and then incubated with a dilution series of Biotin-66008-4 (start concentration 500 ng/ml). And then detected with Streptavidin Poly-HRP (1:2000) followed by incubation with HRP Substrate, terminated with 2M H2SO4, then measuring the resulting absorbance at 450 nm. Negative control is 66008-4-Ig.

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Tested Applications

• Positive IF/ICC detected in: Transfected HEK-293 cells
• Positive ELISA detected in: FusionProtein

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200
• Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:2000-1:4000000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-66008-4 targets DYKDDDDK tag in IF/ICC, ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: DYKDDDDK tag
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.