CoraLite®594-conjugated Fibronectin Monoclonal antibody

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• KD/KO Validated

Fibronectin Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G10F9

Cat no : CL594-66042

Synonyms

FN1, ED B, Cold-insoluble globulin, Cold insoluble globulin, CIG

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Validation Data Gallery

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  IF Staining of human colon cancer using CL594-66042

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 Fibronectin antibody (CL594-66042, Clone: 1G10F9 ) at dilution of 1:200.

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  IF Staining of human colon cancer using CL594-66042

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 Fibronectin antibody (CL594-66042, Clone: 1G10F9 ) at dilution of 1:200.

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  IF Staining of NIH/3T3 using CL594-66042

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CL594-66042 (Fibronectin antibody) at dilution of 1:100.

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  FC experiment of HEK-293 using CL594-66042

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Fibronectin (CL594-66042, Clone:1G10F9) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF-P detected in: human colon cancer tissue
• Positive IF/ICC detected in: NIH/3T3 cells
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66042 targets Fibronectin in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Fibronectin fusion protein Ag8016
• Full Name: fibronectin 1
• Calculated Molecular Weight: 2386 aa, 263 kDa
• Observed Molecular Weight: 263 kDa
• GenBank Accession Number: BC005858
• Gene Symbol: Fibronectin
• Gene ID (NCBI): 2335
• RRID: AB_2883488
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C