CoraLite® Plus 488-conjugated Fibronectin Monoclonal antibody

• Featured Product
• KD/KO Validated

Fibronectin Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

1

CloneNo.

1G10F9

Cat no : CL488-66042

Synonyms

FN1, ED B, Cold-insoluble globulin, Cold insoluble globulin, CIG

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Validation Data Gallery

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  IF Staining of human colon cancer using CL488-66042

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 Fibronectin antibody (CL488-66042, Clone: 1G10F9 ) at dilution of 1:200.

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  IF Staining of human colon cancer using CL488-66042

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 Fibronectin antibody (CL488-66042, Clone: 1G10F9 ) at dilution of 1:200.

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  IF Staining of NIH/3T3 using CL488-66042

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CL488-66042 (Fibronectin antibody) at dilution of 1:100.

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Tested Applications

• Positive IF-P detected in: human colon cancer tissue
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

CL488-66042 targets Fibronectin in IHC, IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Fibronectin fusion protein Ag8016
• Full Name: fibronectin 1
• Calculated Molecular Weight: 2386 aa, 263 kDa
• Observed Molecular Weight: 263 kDa
• GenBank Accession Number: BC005858
• Gene Symbol: Fibronectin
• Gene ID (NCBI): 2335
• RRID: AB_2883236
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)

Publications

Species	Application	Title
mouse	IHC	Front Pharmacol; Ginsenoside Rb1 Ameliorates Diabetic Arterial Stiffening via AMPK Pathway.; Authors - Xinyu Zhang