Fibronectin Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Fibronectin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1G10F9

Cat no : 66042-1-PBS

Synonyms

FN1, ED B, Cold-insoluble globulin, Cold insoluble globulin, CIG

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Validation Data Gallery

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  WB analysis of human plasma using 66042-1-Ig (same clone as 66042-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MG-63 using 66042-1-Ig (same clone as 66042-1-PBS)

  MG-63 cells were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66042-1-Ig (same clone as 66042-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HuH-7 using 66042-1-Ig (same clone as 66042-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 66042-1-Ig (same clone as 66042-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat serum using 66042-1-Ig (same clone as 66042-1-PBS)

  rat serum were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

• 

  IP experiment of human plasma using 66042-1-Ig (same clone as 66042-1-PBS)

  IP result of anti-Fibronectin (IP:66042-1-Ig, 5ug; Detection:66042-1-Ig 1:500) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66042-1-Ig (same clone as 66042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66042-1-Ig (same clone as 66042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Fibronectin antibody (66042-1-Ig, Clone: 1G10F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Fibronectin antibody (66042-1-Ig, Clone: 1G10F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 66042-1-Ig (same clone as 66042-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Fibronectin antibody (66042-1-Ig, Clone: 1G10F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 66042-1-Ig (same clone as 66042-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol ) fixed NIH/3T3 cells using 66042-1-Ig(Fibronectin antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

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Product Information

66042-1-PBS targets Fibronectin in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Fibronectin fusion protein Ag8016
• Full Name: fibronectin 1
• Calculated Molecular Weight: 2386 aa, 263 kDa
• Observed Molecular Weight: 263 kDa
• GenBank Accession Number: BC005858
• Gene Symbol: Fibronectin
• Gene ID (NCBI): 2335
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)