Filter: AllWBIPIHCIF-PIF/ICC

WB analysis of human plasma using 66042-1-Ig (same clone as 66042-1-PBS)
human plasma were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.
WB analysis of MG-63 using 66042-1-Ig (same clone as 66042-1-PBS)
MG-63 cells were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.
WB analysis using 66042-1-Ig (same clone as 66042-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.
WB analysis of HuH-7 using 66042-1-Ig (same clone as 66042-1-PBS)
HuH-7 cells were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.
WB analysis of U2OS using 66042-1-Ig (same clone as 66042-1-PBS)
U2OS cells were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.
WB analysis of rat serum using 66042-1-Ig (same clone as 66042-1-PBS)
rat serum were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.
IP experiment of human plasma using 66042-1-Ig (same clone as 66042-1-PBS)
IP result of anti-Fibronectin (IP:66042-1-Ig, 5ug; Detection:66042-1-Ig 1:500) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 66042-1-Ig (same clone as 66042-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 66042-1-Ig (same clone as 66042-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.
IF Staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Fibronectin antibody (66042-1-Ig, Clone: 1G10F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.
IF Staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Fibronectin antibody (66042-1-Ig, Clone: 1G10F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.
IF Staining of NIH/3T3 using 66042-1-Ig (same clone as 66042-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Fibronectin antibody (66042-1-Ig, Clone: 1G10F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.
IF Staining of NIH/3T3 using 66042-1-Ig (same clone as 66042-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol ) fixed NIH/3T3 cells using 66042-1-Ig(Fibronectin antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Fibronectin Monoclonal antibody, PBS Only

Fibronectin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 66042-1-PBS

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

FN1, ED B, Cold-insoluble globulin, Cold insoluble globulin, CIG

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66042-1-PBS targets Fibronectin in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	Fibronectin fusion protein Ag8016 Predict reactive species
Full Name	fibronectin 1
Calculated Molecular Weight	2386 aa, 263 kDa
Observed Molecular Weight	263 kDa
GenBank Accession Number	BC005858
Gene Symbol	Fibronectin
Gene ID (NCBI)	2335
RRID	AB_11182385
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P02751
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)