FUS/TLS Monoclonal antibody

• Featured Product
• KD/KO Validated

FUS/TLS Monoclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig and More (1)

Applications

WB, IP, IF, RIP, IHC, ELISA

Conjugate

Unconjugated

Publications

21

CloneNo.

3A10B5

Cat no : 60160-1-Ig

Synonyms

75 kDa DNA pairing protein, FUS, FUS CHOP, FUS1, FUS-CHOP, hnRNP P2, Oncogene FUS, Oncogene TLS, POMp75, RNA binding protein FUS, TLS, TLS/CHOP

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Validation Data Gallery

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  WB analysis of HepG2 using 60160-1-Ig

  WB result of FUS/TLS antibody (60160-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FUS/TLS transfected HepG2 cells.

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  WB analysis of HL-60 using 60160-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 60160-1-Ig (FUS/TLS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60160-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60160-1-Ig (FUS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 60160-1-Ig

  IP result of anti-FUS/TLS (IP:60160-1-Ig, 4ug; Detection:60160-1-Ig 1:10000) with HeLa cells lysate 920ug.

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  IP experiment of HeLa using 60160-1-Ig

  HeLa lysates prepared and IP of FUS performed using 1.0 μg of 60160-1-Ig coupled to protein G- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human gliomas using 60160-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60160-1-Ig (FUS/TLS Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 60160-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60160-1-Ig (FUS/TLS antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 60160-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60160-1-Ig (FUS/TLS antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 60160-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60160-1-Ig (FUS/TLS Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 60160-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60160-1-Ig (FUS/TLS Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 60160-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60160-1-Ig (FUS/TLS Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain (FTLD) tissue using 60160-1-Ig

  IHC result of Monoclonal FUS antibody (60160-1-Ig) from Dr. Linda Kwong.

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  IHC staining of human brain (FTLD) tissue using 60160-1-Ig

  IHC result of Monoclonal FUS antibody (60160-1-Ig) from Dr. Linda Kwong.

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  IHC staining of human ovary tumor using 60160-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60160-1-Ig (FUS/TLS antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 60160-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60160-1-Ig (FUS/TLS antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 60160-1-Ig

  HeLa WT cells (yellow outline) and FUS KO cells (red outline) labelled with a green or a far-red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 60160-1-Ig at 1:2000 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IF Staining of human brain(ALS) tissue using 60160-1-Ig

  IF result of McAB FUS (60160-1-Ig) in the Paper "FET proteins TAF15 and EWS are selective markers that distinguish FTLD with FUS pathology from amyotrophic lateral sclerosis with FUS mutations" from Manuela Neumann.

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  IF Staining of HeLa using 60160-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FUS/TLS antibody (60160-1-Ig, Clone: 3A10B5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of human brain(ALS) tissue using 60160-1-Ig

  IF result of McAB FUS (60160-1-Ig) in the Paper "FET proteins TAF15 and EWS are selective markers that distinguish FTLD with FUS pathology from amyotrophic lateral sclerosis with FUS mutations" from Manuela Neumann.

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, HL-60 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human gliomas tissue, human colon tissue, human brain (FTLD) tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 6 publications below
• IF: See 8 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Product Information

60160-1-Ig targets FUS/TLS in WB, IP, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, Drosophila
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FUS/TLS fusion protein Ag2150
• Full Name: fusion (involved in t(12;16) in malignant liposarcoma)
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 68-75 kDa
• GenBank Accession Number: BC026062
• Gene Symbol: FUS/TLS
• Gene ID (NCBI): 2521
• RRID: AB_10666169
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration. Two research groups simultaneously reported that FUS is present in 5% of the pathalogical aggregations (inclusions) seen in familial amyotrophic sclerosis (fALS). FUS-positive inclusions were also reported in cases of sporadic ALS (sALS). More recently, wild-type FUS has also been implicated in the pathological development of frototemporal lobar dementia (FTLD) with ubiquitin-positive inclusions (FTLD-U), further linking FUS to the pathogenesis of neurogenerative diseases. There is some debate as to whether FUS colocalizes with TDP-43 in TDP-43-positive cases of ALS and whether TDP-43 and FUS cause neurodegenerative disease independently or contributively of one another. This antibody is a mouse monoclonal antibody raised against an internal region of human FUS. Initial reports from our customers suggest this new monoclonal FUS antibody (60160-1-Ig) is a useful tool in ALS and FTLD research. For more details, please see our blog article regarding the matter.

Publications

Species	Application	Title
	WB,IF	J Cell Biol; The RNA-binding protein Fus directs translation of localized mRNAs in APC-RNP granules.; Authors - Yasuda Kyota K ; KD Validated
		Brain; Motor neuron degeneration in spastic paraplegia 11 mimics amyotrophic lateral sclerosis lesions.; Authors - Paola S Denora
human	IHC	Acta Neuropathol; Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.; Authors - Neumann Manuela M
human	WB,IF	Ann Neurol; A Novel Missense Mutation of CMT2P Alters Transcription Machinery.; Authors - Bo Hu
human	WB,IF	Cell Rep; A Wnt-induced lncRNA-DGCR5 splicing switch drives tumor-promoting inflammation in esophageal squamous cell carcinoma; Authors - Yue Li
human	IF	Acta Neuropathol Commun; RBM45 associates with nuclear stress bodies and forms nuclear inclusions during chronic cellular stress and in neurodegenerative diseases.; Authors - Mahlon Collins

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