WB analysis using 67881-1-Ig (same clone as 67881-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67881-1-Ig (FMO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67881-1-PBS in a different storage buffer formulation.
Various lysates were subjected to SDS PAGE followed by western blot with 67881-1-Ig (FMO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67881-1-PBS in a different storage buffer formulation.
WB analysis of HuH-7 using 67881-1-Ig (same clone as 67881-1-PBS)
HuH-7 cells were subjected to SDS PAGE followed by western blot with 67881-1-Ig (FMO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67881-1-PBS in a different storage buffer formulation.
HuH-7 cells were subjected to SDS PAGE followed by western blot with 67881-1-Ig (FMO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67881-1-PBS in a different storage buffer formulation.
IF Staining of mouse liver using 67881-1-Ig (same clone as 67881-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67881-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67881-1-PBS in a different storage buffer formulation.
IF Staining of mouse liver using 67881-1-Ig (same clone as 67881-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67881-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67881-1-PBS in a different storage buffer formulation.
FC experiment of HuH-7 using 67881-1-Ig (same clone as 67881-1-PBS)
1X10^6 HuH-7 cells were intracellularly stained with 0.4 ug Anti-Human FMO4 (67881-1-Ig, Clone:2D12E10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67881-1-PBS in a different storage buffer formulation.
1X10^6 HuH-7 cells were intracellularly stained with 0.4 ug Anti-Human FMO4 (67881-1-Ig, Clone:2D12E10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67881-1-PBS in a different storage buffer formulation.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
WB analysis using 67881-1-Ig (same clone as 67881-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67881-1-Ig (FMO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67881-1-PBS in a different storage buffer formulation.
WB analysis of HuH-7 using 67881-1-Ig (same clone as 67881-1-PBS)
HuH-7 cells were subjected to SDS PAGE followed by western blot with 67881-1-Ig (FMO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67881-1-PBS in a different storage buffer formulation.
IF-P Figures
IF Staining of mouse liver using 67881-1-Ig (same clone as 67881-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67881-1-PBS in a different storage buffer formulation.
IF Staining of mouse liver using 67881-1-Ig (same clone as 67881-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67881-1-PBS in a different storage buffer formulation.
FC (INTRA) Figures
FC experiment of HuH-7 using 67881-1-Ig (same clone as 67881-1-PBS)
1X10^6 HuH-7 cells were intracellularly stained with 0.4 ug Anti-Human FMO4 (67881-1-Ig, Clone:2D12E10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67881-1-PBS in a different storage buffer formulation.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
FMO4 Monoclonal antibody
Catalog Number
67881-1-PBS
Citations
-
Dilutions
Applications
WB, IF-P, FC (Intra), Indirect ELISA
Reactivity
human, mouse, rat, rabbit, pig
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
