FMO4 Monoclonal antibody, PBS Only

FMO4 Monoclonal Antibody for WB, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D12E10

Cat no : 67881-1-PBS

Synonyms

Hepatic flavin-containing monooxygenase 4, FMO2, EC:1.14.13.8, Dimethylaniline monooxygenase [N-oxide-forming] 4, 2D12E10

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Validation Data Gallery

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  WB analysis using 67881-1-Ig (same clone as 67881-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67881-1-Ig (FMO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67881-1-PBS in a different storage buffer formulation.

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  WB analysis of HuH-7 using 67881-1-Ig (same clone as 67881-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 67881-1-Ig (FMO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67881-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 67881-1-Ig (same clone as 67881-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67881-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 67881-1-Ig (same clone as 67881-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67881-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HuH-7 using 67881-1-Ig (same clone as 67881-1-PBS)

  1X10^6 HuH-7 cells were intracellularly stained with 0.4 ug Anti-Human FMO4 (67881-1-Ig, Clone:2D12E10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67881-1-PBS in a different storage buffer formulation.

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Product Information

67881-1-PBS targets FMO4 in WB, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

• Tested Reactivity: Human, mouse, rat, rabbit, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FMO4 fusion protein Ag7935
• Full Name: flavin containing monooxygenase 4
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC002780
• Gene Symbol: FMO4
• Gene ID (NCBI): 2329
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.