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FMO4 Monoclonal antibody

FMO4 Monoclonal Antibody for WB, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2D12E10

Cat no : 67881-1-Ig

Synonyms

Hepatic flavin-containing monooxygenase 4, FMO2, EC:1.14.13.8, Dimethylaniline monooxygenase [N-oxide-forming] 4, 2D12E10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FMO4 Monoclonal antibody (67881-1-Ig)

    WB analysis using 67881-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67881-1-Ig (FMO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HuH-7 cells using FMO4 Monoclonal antibody (67881-1-Ig)

    WB analysis of HuH-7 using 67881-1-Ig

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 67881-1-Ig (FMO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using FMO4 Monoclonal antibody (67881-1-Ig)

    IF Staining of mouse liver using 67881-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using FMO4 Monoclonal antibody (67881-1-Ig)

    IF Staining of mouse liver using 67881-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HuH-7 cells using FMO4 Monoclonal antibody (67881-1-Ig)

    FC experiment of HuH-7 using 67881-1-Ig

    1X10^6 HuH-7 cells were intracellularly stained with 0.4 ug Anti-Human FMO4 (67881-1-Ig, Clone:2D12E10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inrabbit liver tissue, HuH-7 cells, pig liver tissue, rat liver tissue, mouse liver tissue
Positive IF-P detected inmouse liver tissue
Positive FC (Intra) detected inHuH-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67881-1-Ig targets FMO4 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Reactivity Human, mouse, rat, rabbit, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FMO4 fusion protein Ag7935
Full Name flavin containing monooxygenase 4
Calculated Molecular Weight 63 kDa
Observed Molecular Weight63 kDa
GenBank Accession NumberBC002780
Gene Symbol FMO4
Gene ID (NCBI) 2329
RRIDAB_2918638
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FMO4 antibody 67881-1-IgDownload protocol
IF protocol for FMO4 antibody 67881-1-IgDownload protocol
FC protocol for FMO4 antibody 67881-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols