Various lysates were subjected to SDS PAGE followed by western blot with 67881-1-Ig (FMO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 67881-1-Ig (FMO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis of HuH-7 using 67881-1-Ig
HuH-7 cells were subjected to SDS PAGE followed by western blot with 67881-1-Ig (FMO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
HuH-7 cells were subjected to SDS PAGE followed by western blot with 67881-1-Ig (FMO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IF Staining of mouse liver using 67881-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
IF Staining of mouse liver using 67881-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
FC experiment of HuH-7 using 67881-1-Ig
1X10^6 HuH-7 cells were intracellularly stained with 0.4 ug Anti-Human FMO4 (67881-1-Ig, Clone:2D12E10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 HuH-7 cells were intracellularly stained with 0.4 ug Anti-Human FMO4 (67881-1-Ig, Clone:2D12E10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
Various lysates were subjected to SDS PAGE followed by western blot with 67881-1-Ig (FMO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis of HuH-7 using 67881-1-Ig
HuH-7 cells were subjected to SDS PAGE followed by western blot with 67881-1-Ig (FMO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IF-P Figures
IF Staining of mouse liver using 67881-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
IF Staining of mouse liver using 67881-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
FC (INTRA) Figures
FC experiment of HuH-7 using 67881-1-Ig
1X10^6 HuH-7 cells were intracellularly stained with 0.4 ug Anti-Human FMO4 (67881-1-Ig, Clone:2D12E10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
FMO4 Monoclonal antibody
Catalog Number
67881-1-Ig
Citations
-
Dilutions
WB : 1:5000-1:50000 IF-P : 1:200-1:800 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IF-P, FC (Intra), ELISA
Reactivity
human, mouse, rat, rabbit, pig
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
