FKBPL Monoclonal antibody, PBS Only

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  WB analysis using 66389-1-Ig (same clone as 66389-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66389-1-Ig (FKBPL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66389-1-PBS in a different storage buffer formulation.

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  WB analysis using 66389-1-Ig (same clone as 66389-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66389-1-Ig (FKBPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66389-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66389-1-Ig (same clone as 66389-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66389-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66389-1-Ig (same clone as 66389-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66389-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66389-1-Ig (same clone as 66389-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66389-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66389-1-Ig (same clone as 66389-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66389-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66389-1-Ig (same clone as 66389-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66389-1-Ig (FKBPL antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66389-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66389-1-Ig (same clone as 66389-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug FKBPL Monoclonal antibody (66389-1-Ig, Clone:2H10B10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66389-1-PBS in a different storage buffer formulation.

FKBPL Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 66389-1-PBS

Clone No.2H10B10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

WISp39, WAF-1/CIP1 stabilizing protein 39, NG7, FK506-binding protein-like, DIR1

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Product Information

66389-1-PBS targets FKBPL in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FKBPL fusion protein Ag0112
• Full Name: FK506 binding protein like
• Calculated Molecular Weight: 349 aa, 38 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC004168
• Gene Symbol: FKBPL
• Gene ID (NCBI): 63943
• RRID: AB_2881765
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UIM3
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

FKBPL, also named as DIR1, NG7 and WISp39, has similarity to the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBPL levels may be a prognostic indicator and determinant of response to endocrine therapy(PMID:20103631, 15664193) .It can be detected the band between 38 kDa and 48 kDa by western blot.