Various lysates were subjected to SDS PAGE followed by western blot with 66389-1-Ig (FKBPL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 66389-1-Ig (FKBPL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 66389-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66389-1-Ig (FKBPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 66389-1-Ig (FKBPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 66389-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 66389-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 66389-1-Ig
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of MCF-7 using 66389-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66389-1-Ig (FKBPL antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66389-1-Ig (FKBPL antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
FC experiment of HeLa using 66389-1-Ig
1x10^6 HeLa cells were intracellularly stained with 0.4 ug FKBPL Monoclonal antibody (66389-1-Ig, Clone:2H10B10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 HeLa cells were intracellularly stained with 0.4 ug FKBPL Monoclonal antibody (66389-1-Ig, Clone:2H10B10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human breast cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
MCF-7 cells
Positive FC (Intra) detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:10000
Immunohistochemistry (IHC)
IHC : 1:100-1:400
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
FKBPL, also named as DIR1, NG7 and WISp39, has similarity to the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBPL levels may be a prognostic indicator and determinant of response to endocrine therapy(PMID:20103631, 15664193) .It can be detected the band between 38 kDa and 48 kDa by western blot.
Various lysates were subjected to SDS PAGE followed by western blot with 66389-1-Ig (FKBPL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 66389-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66389-1-Ig (FKBPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human breast cancer using 66389-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 66389-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 66389-1-Ig
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human testis using 66389-1-Ig
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 40x lens).
IF/ICC Figures
IF Staining of MCF-7 using 66389-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66389-1-Ig (FKBPL antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
FC (INTRA) Figures
FC experiment of HeLa using 66389-1-Ig
1x10^6 HeLa cells were intracellularly stained with 0.4 ug FKBPL Monoclonal antibody (66389-1-Ig, Clone:2H10B10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
FKBPL Monoclonal antibody
Catalog Number
66389-1-Ig
Citations
1
Dilutions
WB : 1:2000-1:10000 IHC : 1:100-1:400 IF/ICC : 1:50-1:500 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
