CoraLite®594-conjugated FBF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

FBF1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-11531

Synonyms

Alb, albatross, Fas (TNFRSF6) binding factor 1, FBF 1, FBF1, FBF1 Ens, FLJ00103, FLJ13996, HCG1989313, isoform CRA_b, KIAA1863

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Validation Data Gallery

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  IF Staining of A549 using CL594-11531

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite®594 FBF1 antibody (CL594-11531) at dilution of 1:200.

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  IF Staining of HUVEC using CL594-11531

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite®594 FBF1 antibody (CL594-11531) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: A549 cells, HUVEC cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-11531 targets FBF1 in IF/ICC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBF1 fusion protein Ag2106
• Full Name: Fas (TNFRSF6) binding factor 1
• Calculated Molecular Weight: 1133 aa, 125 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC023549
• Gene Symbol: FBF1
• Gene ID (NCBI): 85302
• RRID: AB_2919767
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

FBF1, also named as ALB and KIAA1863, is keratin-binding protein required for epithelial cell polarization. It is involved in apical junction complex (AJC) assembly via its interaction with PARD3. FBF1 is required for ciliogenesis. FBF1 has six isoforms with MW 125-127 kDa, 99 kDa, 69 kDa and 55 kDa. This antibody is induced by N-terminal (20-347aa) of FBF1. It recognizes all the six isoforms.