FBF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

FBF1 Polyclonal Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

27

Cat no : 11531-1-AP

Synonyms

FBF-1, FBF 1, Fas-binding factor 1, Fas (TNFRSF6) binding factor 1, albatross

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Validation Data Gallery

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  IF Staining of A549 using 11531-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using FBF1 antibody (11531-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HUVEC using 11531-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using FBF1 antibody (11531-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IF/ICC detected in: A549 cells, HUVEC cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 23 publications below
• IP: See 1 publications below

Product Information

11531-1-AP targets FBF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBF1 fusion protein Ag2106
• Full Name: Fas (TNFRSF6) binding factor 1
• Calculated Molecular Weight: 1133 aa, 125 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC023549
• Gene Symbol: FBF1
• Gene ID (NCBI): 85302
• RRID: AB_2246683
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBF1, also named as ALB and KIAA1863, is keratin-binding protein required for epithelial cell polarization. It is involved in apical junction complex (AJC) assembly via its interaction with PARD3. FBF1 is required for ciliogenesis. FBF1 has six isoforms with MW 125-127 kDa, 99 kDa, 69 kDa and 55 kDa. This antibody is induced by N-terminal (20-347aa) of FBF1. It recognizes all the six isoforms.

Publications

Species	Application	Title
human	WB,IF	Nat Commun; A stress-induced cilium-to-PML-NB route drives senescence initiation; Authors - Xiaoyu Ma
human	WB,IF	Nat Commun; Transiently formed nucleus-to-cilium microtubule arrays mediate senescence initiation in a KIFC3-dependent manner; Authors - Jielu Hao Robichaud
human	IF	Sci Adv; Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.; Authors - Chuan Chen
human	IF	Nat Commun; Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules.; Authors - Kai He
human	IF	Nat Commun; A distal centriolar protein network controls organelle maturation and asymmetry.; Authors - Lei Wang
human	IF,WB	Nat Commun; TALPID3 and ANKRD26 selectively orchestrate FBF1 localization and cilia gating.; Authors - Hao Yan