Tested Applications
| Positive IF/ICC detected in | A549 cells, HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11531-1-AP targets FBF1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2106 Product name: Recombinant human FBF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 20-347 aa of BC023549 Sequence: LASHTRDTTGVSQMFPSSKARTKSLLGDDVFSTMAGLEEADAEVSVISEADPQALLQAMKDLDGMDADILGLKKSNSAPSKKAAKDPGKGELPNHPKPAGGAIPTKKSLPSPSSSGHQNRRFSSEDLEDPLGGLLSYDEGGITKQPPVTQSKTASDKSPSTVRDQGPSIPLTPGDTPIRKKEELLFDDGDDIMATLGFGDSPKAEKRQIGDQEGPRPARSTLDELLGRGMATKLLARPGTGEHREFKLDKKYQRPQDSEDMWGDEDFTFGAYQPTVVSSEGRQSRRQSVSRFFADSGADPKGEPGSKQSPPMASSPIQPRKGGADWLG Predict reactive species |
| Full Name | Fas (TNFRSF6) binding factor 1 |
| Calculated Molecular Weight | 1133 aa, 125 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC023549 |
| Gene Symbol | FBF1 |
| Gene ID (NCBI) | 85302 |
| RRID | AB_2246683 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TES7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FBF1, also named as ALB and KIAA1863, is keratin-binding protein required for epithelial cell polarization. It is involved in apical junction complex (AJC) assembly via its interaction with PARD3. FBF1 is required for ciliogenesis. FBF1 has six isoforms with MW 125-127 kDa, 99 kDa, 69 kDa and 55 kDa. This antibody is induced by N-terminal (20-347aa) of FBF1. It recognizes all the six isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FBF1 antibody 11531-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-FBF1 antibody (Cat# 11531-1-AP) has 29 citations across journals including Nature Communications (7), eLife (3), Journal of Cell Biology (2), Cell Reports (2), Genes & Cells (2), ACS Nano, Advanced Science, Science Advances, Developmental Cell, PLoS Genetics, and others. Research fields span ciliogenesis, centriole biology, intraflagellar transport, cell senescence, photoreceptor function, stem cell biology, and liver pathology.
| Species | Application | Title |
|---|---|---|
Nat Commun Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules. | ||
Nat Commun A distal centriolar protein network controls organelle maturation and asymmetry. | ||
Nat Commun Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis. | ||
Nat Commun Transition fibre protein FBF1 is required for the ciliary entry of assembled intraflagellar transport complexes.
| ||
Nat Commun Transiently formed nucleus-to-cilium microtubule arrays mediate senescence initiation in a KIFC3-dependent manner | ||
