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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated FBF1 Polyclonal antibody

FBF1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11531

Synonyms

Alb, albatross, Fas (TNFRSF6) binding factor 1, FBF 1, FBF1, FBF1 Ens, FLJ00103, FLJ13996, HCG1989313, isoform CRA_b, KIAA1863

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite® Plus 488-conjugated FBF1 Polyclonal anti (CL488-11531)

    IF Staining of A549 using CL488-11531

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 FBF1 antibody (CL488-11531) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using CoraLite® Plus 488-conjugated FBF1 Polyclonal anti (CL488-11531)

    IF Staining of HUVEC using CL488-11531

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite® Plus 488 FBF1 antibody (CL488-11531) at dilution of 1:200.

Tested Applications

Positive IF/ICC detected inA549 cells, HUVEC cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11531 targets FBF1 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBF1 fusion protein Ag2106
Full Name Fas (TNFRSF6) binding factor 1
Calculated Molecular Weight1133 aa, 125 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC023549
Gene Symbol FBF1
Gene ID (NCBI) 85302
RRIDAB_2919031
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FBF1, also named as ALB and KIAA1863, is keratin-binding protein required for epithelial cell polarization. It is involved in apical junction complex (AJC) assembly via its interaction with PARD3. FBF1 is required for ciliogenesis. FBF1 has six isoforms with MW 125-127 kDa, 99 kDa, 69 kDa and 55 kDa. This antibody is induced by N-terminal (20-347aa) of FBF1. It recognizes all the six isoforms.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 FBF1 antibody CL488-11531Download protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Karine (Verified Customer) (02-26-2024)

FBF1-Coralite488 (in green) does label one extremity of the primary cilium labeled with ARL13B (in red) in RPE1 cells whose nuclei appear in purple and cell contour in grey with Brightfield. It is quite specific when used at 1/1000 overnight at 4°C. There is a bit more aspecific signal than with the uncoupled antibdy but it remains quite reasonable.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: RPE1 cells
CL Plus 488 FBF1 Antibody Immunofluorescence validation (1/1000 dilution) in RPE1 cells (Cat no:CL488-11531)