EZH2 Recombinant antibody, PBS Only (Capture)

EZH2 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242309F11

Cat no : 84824-2-PBS

Synonyms

EZH1, ENX-1, ENX 1, Enhancer of zeste homolog 2, EC:2.1.1.356

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Validation Data Gallery

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  WB analysis using 84824-2-RR (same clone as 84824-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84824-2-RR (EZH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84824-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84824-2-RR (same clone as 84824-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EZH2 antibody (84824-2-RR, Clone: 242309F11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84824-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01605-1

  Cytometric bead array standard curve of MP01605-1, EZH2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84824-2-PBS. Detection antibody: 84824-3-PBS. Standard: Ag16789. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP01605-2

  Cytometric bead array standard curve of MP01605-2, EZH2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84824-2-PBS. Detection antibody: 84824-1-PBS. Standard: Ag16789. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84824-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84824-2-RR against Human EZH2 were performed. The affinity constant is 2.73 nM.

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Product Information

84824-2-PBS targets EZH2 as part of a matched antibody pair:

MP01605-1: 84824-2-PBS capture and 84824-3-PBS detection (validated in Cytometric bead array)

MP01605-2: 84824-2-PBS capture and 84824-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: EZH2 fusion protein Ag16789
• Full Name: enhancer of zeste homolog 2 (Drosophila)
• Calculated Molecular Weight: 751 aa, 86 kDa
• Observed Molecular Weight: 90-102 kDa
• GenBank Accession Number: BC010858
• Gene Symbol: EZH2
• Gene ID (NCBI): 2146
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)