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EZH2 Recombinant antibody

EZH2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242309F11

Cat no : 84824-2-RR

Synonyms

EZH1, ENX-1, ENX 1, Enhancer of zeste homolog 2, EC:2.1.1.356

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EZH2 Recombinant antibody (84824-2-RR)

    WB analysis using 84824-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84824-2-RR (EZH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EZH2 Recombinant antibody (84824-2-RR)

    IF Staining of HepG2 using 84824-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EZH2 antibody (84824-2-RR, Clone: 242309F11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84824-2-RR

    Affinity and Kinetic Characterization of 84824-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84824-2-RR against Human EZH2 were performed. The affinity constant is 2.73 nM.

Tested Applications

Positive WB detected inJurkat cells, Raji cells, NIH/3T3 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84824-2-RR targets EZH2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EZH2 fusion protein Ag16789
Full Name enhancer of zeste homolog 2 (Drosophila)
Calculated Molecular Weight 751 aa, 86 kDa
Observed Molecular Weight 90-102 kDa
GenBank Accession NumberBC010858
Gene Symbol EZH2
Gene ID (NCBI) 2146
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)

Protocols

Product Specific Protocols
WB protocol for EZH2 antibody 84824-2-RRDownload protocol
IF protocol for EZH2 antibody 84824-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols