EZH2 Recombinant antibody

EZH2 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242309F11

Cat no : 84824-2-RR

Synonyms

EZH1, ENX-1, ENX 1, Enhancer of zeste homolog 2, EC:2.1.1.356

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Validation Data Gallery

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  WB analysis using 84824-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84824-2-RR (EZH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 84824-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EZH2 antibody (84824-2-RR, Clone: 242309F11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84824-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84824-2-RR against Human EZH2 were performed. The affinity constant is 2.73 nM.

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Tested Applications

• Positive WB detected in: Jurkat cells, Raji cells, NIH/3T3 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84824-2-RR targets EZH2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: EZH2 fusion protein Ag16789
• Full Name: enhancer of zeste homolog 2 (Drosophila)
• Calculated Molecular Weight: 751 aa, 86 kDa
• Observed Molecular Weight: 90-102 kDa
• GenBank Accession Number: BC010858
• Gene Symbol: EZH2
• Gene ID (NCBI): 2146
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)