CoraLite® Plus 488-conjugated EYA1 Polyclonal antibody

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  IF Staining of HeLa using CL488-22658

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 EYA1 antibody (CL488-22658) at dilution of 1:200.

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  FC experiment of HeLa using CL488-22658

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Eya1 Polyclonal Antibody (CL488-22658)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• Featured Product
• KD/KO Validated

EYA1 Polyclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL488-22658

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

EC:3.1.3.16, BOR, BOP

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-22658 targets EYA1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EYA1 fusion protein Ag18489
• Full Name: eyes absent homolog 1 (Drosophila)
• Calculated Molecular Weight: 592 aa, 65 kDa
• Observed Molecular Weight: 60 kDa, 50 kDa
• GenBank Accession Number: BC121799
• Gene Symbol: EYA1
• Gene ID (NCBI): 2138
• RRID: AB_3672747
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99502
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The EYA1 (Eyes absent homolog 1) gene is the human homologue of the Drosophila Eya1 gene, which is essential for eye development in that species. There are 4 different isoforms of EYA1 (EYA1A, EYA1B, EYA1C, EYA1D), which are composed of 559 amino acids (AA), 592 AA, 592 AA and 557 AA, respectively. It should be noted that isoforms B and C have the same length; however, they could be considered different variants because of change in amino acidic composition (PMID: 24803398).