CoraLite®594-conjugated ESRRB Monoclonal antibody

ESRRB Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3E10C1

Cat no : CL594-66818

Synonyms

ERR-beta, ERRbeta, ERRB2, ERRb, ERR beta-2

---

Validation Data Gallery

• 

  IF Staining of Caco-2 using CL594-66818

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CoraLite®594 ESRRB antibody (CL594-66818, Clone: 3E10C1 ) at dilution of 1:600.

• 

  FC experiment of Caco-2 using CL594-66818

  1x10^6 Caco-2 cells were intracellularly stained with 0.4 ug Coralite®594 ESRRB Monoclonal Antibody (CL594-66818, Clone:3E10C1)(red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (1F8D3) (CL594-66360-1, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Tested Applications

• Positive IF/ICC detected in: Caco-2 cells
• Positive FC (Intra) detected in: Caco-2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66818 targets ESRRB in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ESRRB fusion protein Ag18626
• Full Name: estrogen-related receptor beta
• Calculated Molecular Weight: 508 aa, 56 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC131517
• Gene Symbol: ESRRB
• Gene ID (NCBI): 2103
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Estrogen related receptor beta (ESRRB), a NR3 Steroid Receptor, has been shown to affect early placental development. Also it can act as a repressor of transcriptional activity mediated by the glucocorticoid receptor (GR). It binds as a monomer to the extended half-site TNAAGTGCA and as a homodimer to the estrogen response element (ERE), palindromic thyroid hormone response element (TRE(pal)), and SF-1 response element, but not to the glucocorticoid response element (GRE). Estrogen related receptor beta mutations lead to abnormal development of the chorion and defective diploid trophoblast proliferation, and are lethal during midgestation. Expression has been documented in mice in the extra embryonic ectoderm that gives rise to the chorion. ESTs have been isolated from normal human eye, lung, and testis libraries. The ligand for the receptor is PPARgamma coactivator 1 (PGC-1) beta. ESRRB exists various isoforms and molecular weight of each isoform is 48 kDa,55 kDa and 56 kDa.