ESRRB Monoclonal antibody

ESRRB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3E10C1

Cat no : 66818-1-Ig

Synonyms

ERR-beta, ERRbeta, ERRB2, ERRb, ERR beta-2

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Validation Data Gallery

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  WB analysis using 66818-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66818-1-Ig (ESRRB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66818-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66818-1-Ig (ESRRB antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 66818-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66818-1-Ig (ESRRB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66818-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66818-1-Ig (ESRRB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Caco-2 using 66818-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using ESRRB antibody (66818-1-Ig, Clone: 3E10C1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), Alpha Tubulin antibody (80762-1-RR, Clone: 1G7, red).

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  IF Staining of Caco-2 using 66818-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using ESRRB antibody (66818-1-Ig, Clone: 3E10C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, NCCIT cells, JAR cells, A549 cells, Caco-2 cells, LNCaP cells, HT-29 cells, Rat brain tissue, Mouse brain tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

66818-1-Ig targets ESRRB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ESRRB fusion protein Ag18626
• Full Name: estrogen-related receptor beta
• Calculated Molecular Weight: 508 aa, 56 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC131517
• Gene Symbol: ESRRB
• Gene ID (NCBI): 2103
• RRID: AB_2882161
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Estrogen related receptor beta (ESRRB), a NR3 Steroid Receptor, has been shown to affect early placental development. Also it can act as a repressor of transcriptional activity mediated by the glucocorticoid receptor (GR). It binds as a monomer to the extended half-site TNAAGTGCA and as a homodimer to the estrogen response element (ERE), palindromic thyroid hormone response element (TRE(pal)), and SF-1 response element, but not to the glucocorticoid response element (GRE). Estrogen related receptor beta mutations lead to abnormal development of the chorion and defective diploid trophoblast proliferation, and are lethal during midgestation. Expression has been documented in mice in the extra embryonic ectoderm that gives rise to the chorion. ESTs have been isolated from normal human eye, lung, and testis libraries. The ligand for the receptor is PPARgamma coactivator 1 (PGC-1) beta. ESRRB exists various isoforms and molecular weight of each isoform is 48 kDa,55 kDa and 56 kDa.

Publications

Species	Application	Title
human	IF	Stem Cell Res; Establishment of a CRISPR/Cas9-mediated GATAD2B homozygous knockout human embryonic stem cell line; Authors - Bo Wang