EEA1 Monoclonal antibody

EEA1 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1E11E4

Cat no : 68065-1-Ig

Synonyms

early endosome antigen 1, EEA1, MST105, MSTP105, ZFYVE2



Tested Applications

Positive WB detected inA431 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:1500-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 5 publications below

Product Information

68065-1-Ig targets EEA1 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EEA1 fusion protein Ag28814
Full Name early endosome antigen 1
Calculated Molecular Weight 162 kDa
Observed Molecular Weight 162 kDa
GenBank Accession NumberNM_003566
Gene Symbol EEA1
Gene ID (NCBI) 8411
RRIDAB_2918806
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.

Protocols

Product Specific Protocols
WB protocol for EEA1 antibody 68065-1-IgDownload protocol
IF protocol for EEA1 antibody 68065-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Life Sci Alliance

ACE2-EGFR-MAPK signaling contributes to SARS-CoV-2 infection

Authors - Melanie Engler
humanIF

J Alzheimers Dis

High Fat Diet Mediates Amyloid-β Cleaving Enzyme 1 Phosphorylation and SUMOylation, Enhancing Cognitive Impairment in APP/PS1 Mice

Authors - Jian Bao
mouseIF

Antioxidants (Basel)

Oxybaphus himalaicus Mitigates Lipopolysaccharide-Induced Acute Kidney Injury by Inhibiting TLR4/MD2 Complex Formation

Authors - Honghong Zhan
humanIF

Cell Chem Biol

Targeting VPS41 induces methuosis and inhibits autophagy in cancer cells

Authors - Yue Liu
humanIF

Am J Physiol Cell Physiol

Progranulin and EGFR modulate receptor-like tyrosine kinase sorting and stability in mesothelioma cells

Authors - Elisa Ventura