CoraLite® Plus 488-conjugated EEA1 Monoclonal antibody

EEA1 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1


Human, Mouse, Rat


IF, FC (Intra)


CoraLite® Plus 488 Fluorescent Dye



Cat no : CL488-68065


early endosome antigen 1, EEA1, MST105, MSTP105, ZFYVE2

Tested Applications

Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68065 targets EEA1 in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EEA1 fusion protein Ag28814
Full Name early endosome antigen 1
Calculated Molecular Weight 162 kDa
Observed Molecular Weight 162 kDa
GenBank Accession NumberNM_003566
Gene Symbol EEA1
Gene ID (NCBI) 8411
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.


Product Specific Protocols
IF protocol for CL Plus 488 EEA1 antibody CL488-68065Download protocol
FC protocol for CL Plus 488 EEA1 antibody CL488-68065Download protocol
Standard Protocols
Click here to view our Standard Protocols