E-selectin/CD62E Recombinant antibody, PBS Only (Detector)

E-selectin/CD62E Uni-rAb^TM Recombinant Antibody for FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241767H6

Cat no : 98189-1-PBS

Synonyms

CD62E, E-selectin, ELAM 1, ELAM, E selectin

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP01636-3

  Sandwich ELISA standard curve of MP01636-3, Human E-selectin / CD62E Recombinant Matched Antibody Pair - PBS only. 84887-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02341. 98189-1-PBS was HRP conjugated as the detection antibody. Range: 9.77-625 pg/mL

• 

  Sample test of MP01636-3

  Serum of eight individual healthy human donors was measured. The human E-selectin / CD62E concentration of detected samples was determined to be 12.99 ng/mL with a range of 4.01 - 24.83 ng/mL.

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  FC experiment of HUVEC using 98189-1-RR (same clone as 98189-1-PBS)

  1x10^6 untreated or TNF alpha treated HUVEC cells were surface stained with 0.25 ug Anti-Human E-selectin/CD62E Rabbit Recombinant Antibody (98189-1-RR, Clone: 241767H6) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98189-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HUVEC using 98189-1-RR (same clone as 98189-1-PBS)

  1x10^6 TNF alpha treated HUVEC cells were surface stained with 0.25 ug Anti-Human E-selectin/CD62E Rabbit Recombinant Antibody (98189-1-RR, Clone: 241767H6) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated HUVEC cells were surface stained with 0.25 ug Anti-Human E-selectin/CD62E Rabbit Recombinant Antibody (98189-1-RR, Clone: 241767H6) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed. This data was developed using the same antibody clone with 98189-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01636-1

  Cytometric bead array standard curve of MP01636-1, E-selectin / CD62E Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84887-2-PBS. Detection antibody: 98189-1-PBS. Standard: RP02341. Range: 0.313-40 ng/mL.

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Product Information

98189-1-PBS targets E-selectin/CD62E as part of a matched antibody pair:

MP01636-1: 84887-2-PBS capture and 98189-1-PBS detection (validated in Cytometric bead array)

MP01636-3: 84887-3-PBS capture and 98189-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: selectin E
• Calculated Molecular Weight: 610 aa, 67 kDa
• GenBank Accession Number: BC142711
• Gene Symbol: CD62E
• Gene ID (NCBI): 6401
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

E-selectin, also known as CD62E or ELAM-1, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)